Multiple locus VNTR fingerprinting (MLVF) of Streptococcus pyogenes
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Published on: July 15, 2011
Katarzyna Obszańska1, Anna L Borek, Waleria Hryniewicz
1Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Warszawa, Poland.
Abstract:
Streptococcus pyogenes (GAS) is a human pathogen that causes millions of infections worldwide. Comparison between GAS strains isolated in different laboratories all over the world is often difficult. Three usually used methods have either low resolution (emm typing), are expensive (MLST) or time- and labor-consuming (PFGE). Our laboratory recently developed new, inexpensive methods of GAS typing-VF (virulence factor profiling) and PP (phage profiling). To improve the typing scheme developed for GAS, we recently proposed a new typing method. Here we present detailed protocol for MLVF analysis.
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