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Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation
Published on: January 22, 2020
Dynamic alterations in the expression and localization of ACTL7a during capacitation in mouse spermatozoa
Jun Fu1, Wei Song, Shudong Zong
1State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Tsinghua University, Beijing, People's Republic of China.
Objective:
To demonstrate that capacitation in mouse spermatozoa involves alterations in the expression and localization of ACTL7a.
Design:
Determine the alteration in the expression level and localization of ACTL7a in the induction of capacitation in mouse spermatozoa.
Setting:
The Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, People's Republic of China.
Animal(S):
ICR (Institute of Cancer Research) mice.
Intervention(S):
None.
Main Outcome Measure(S):
Western blot, indirect immunostaining.
Result(S):
The expression of ACTL7a is upregulated via the PKA pathway and undergoes remodeling during the early period of capacitation in mouse spermatozoa.
Conclusion(S):
ACTL7a is an essential component of capacitation in mouse spermatozoa. The alteration in the expression and localization of ACTL7a may be the primary biochemical event in the induction of capacitation in mouse spermatozoa.
