Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cleavage and Blastulation01:33

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical embryology in the United States: current landscape and considerations for professional development and standardization.

Fertility and sterility·2026
Same author

Elevated lipid levels and altered semen parameters in men of couples seeking fertility care.

American journal of epidemiology·2026
Same author

Supplement use among couples seeking fertility treatment and associations with live birth and pregnancy loss.

Fertility and sterility·2026
Same author

Reproductive outcomes of fresh vs. freeze-all embryo transfer strategies with blastocysts stratified using body mass index.

Fertility and sterility·2025
Same author

Ongoing surveillance of analytical platforms in preimplantation genetic testing: phase 4.

Fertility and sterility·2025
Same author

scRNA-seq Can Identify Different Cell Populations in Ovarian Cancer Bulk RNA-seq Experiments.

International journal of molecular sciences·2025

Related Experiment Video

Updated: May 16, 2026

In ovo Expression of MicroRNA in Ventral Chick Midbrain
09:19

In ovo Expression of MicroRNA in Ventral Chick Midbrain

Published on: September 16, 2013

MicroRNA expression in the human blastocyst.

Evan M Rosenbluth1, Dawne N Shelton, Amy E T Sparks

  • 1Department of Obstetrics & Gynecology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA.

Fertility and Sterility
|December 6, 2012
PubMed
Summary

Human blastocysts express microRNAs (miRNAs) critical for development. Differential miRNA expression was observed between sexes and in euploid versus aneuploid embryos, suggesting potential prognostic value.

More Related Videos

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
12:09

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
14:08

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Published on: January 26, 2013

Related Experiment Videos

Last Updated: May 16, 2026

In ovo Expression of MicroRNA in Ventral Chick Midbrain
09:19

In ovo Expression of MicroRNA in Ventral Chick Midbrain

Published on: September 16, 2013

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
12:09

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
14:08

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Published on: January 26, 2013

Area of Science:

  • Reproductive biology
  • Molecular genetics
  • Embryology

Background:

  • MicroRNAs (miRNAs) play crucial roles in gene regulation.
  • Understanding miRNA expression in human blastocysts is vital for reproductive medicine.

Purpose of the Study:

  • Identify highly expressed miRNAs in human blastocysts.
  • Compare miRNA profiles between euploid and aneuploid embryos.
  • Investigate miRNA differences between male and female embryos.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qPCR) was used to analyze miRNA expression in individual human blastocysts.
  • Initial analysis of 14 blastocysts was expanded to 27 blastocysts for confirmation.

Main Results:

  • miR-372 was the most highly expressed miRNA in euploid embryos.
  • Several miRNAs critical for stem cell pluripotency and embryo development were identified.
  • Differential miRNA expression was found based on embryonic sex and chromosomal status.

Conclusions:

  • Human blastocysts express miRNAs essential for survival and development.
  • Sex-based miRNA differences indicate early differentiation.
  • Embryonic miRNA profiles may predict euploid/aneuploid status and developmental potential.