Related Experiment Video
Updated: May 16, 2026

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds
Published on: October 4, 2021
Review: role of carbon sources for in vitro plant growth and development
Mehwish Yaseen1, Touqeer Ahmad, Gaurav Sablok
1Plant Tissue Culture Laboratory, Department of Horticulture, PMAS-Arid Agriculture University, Rawalpindi, Pakistan.
Abstract:
In vitro plant cells, tissues and organ cultures are not fully autotrophic establishing a need for carbohydrates in culture media to maintain the osmotic potential, as well as to serve as energy and carbon sources for developmental processes including shoot proliferation, root induction as well as emission, embryogenesis and organogenesis, which are highly energy demanding developmental processes in plant biology. A variety of carbon sources (both reducing and non-reducing) are used in culture media depending upon genotypes and specific stages of growth. However, sucrose is most widely used as a major transport-sugar in the phloem sap of many plants. In micropropagation systems, morphogenetic potential of plant tissues can greatly be manipulated by varying type and concentration of carbon sources. The present article reviews the past and current findings on carbon sources and their sustainable utilization for in vitro plant tissue culture to achieve better growth rate and development.
Related Concept Videos
Cell Culture
Designing Growth Media for Bioreactors
The Roles of Bacteria and Fungi in Plant Nutrition
Key Elements for Plant Nutrition
Plant Tissue Culture
Meristems and Plant Growth
