Cytotoxic tirucallane triterpenoids from the stem bark of Melia toosendan
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Updated: May 16, 2026

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine
Published on: February 24, 2023
Qingjie Zhao1, Yan Song, Chuanwei Feng
1Department of Organic Chemistry, College of Pharmacy, Second Military Medical University, Shanghai 200433, China. qjzhao@smmu.edu.cn
Abstract:
Three new tirucallane triterpenoids, 3β,16-dihydroxy-25-hydroperoxytirucalla-7,23(24)-dien-6-oxo acid (1), 3β,16β-hydroxytirucalla-7,24(25)-dien-21,23-olide (2), and 3β,16β-hydroxytirucalla-7,24(25)-dien-6-oxo-21,23-olide (3) were isolated from the 70% ethanol extract of the stem bark of Melia toosendan. Their structures were determined on the basis of 1D and 2D NMR (COSY, HMQC, HMBC and NOESY) analyses. The isolated compounds were evaluated in vitro for cytotoxic activities against A549, SK-OV-3, SK-MEL-2 and HCT15 tumor cell lines. Compounds 2 and 3 exhibited significant cytotoxic activities against the four tested cell lines with IC(50) values of 3.4-5.7 μg/mL and 3.2-5.0 μg/mL, respectively.
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