Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced Liver Fibrosis Test, FIB-4 and FibroScan: Real-World Prognostic Accuracy for MASLD in a Biopsy-Controlled Cohort.

Liver international : official journal of the International Association for the Study of the Liver·2026
Same author

Longitudinal Trends of Salivary Oxidized Thymosin β4 and β10 in Preterm Infants with Bronchopulmonary Dysplasia.

Children (Basel, Switzerland)·2026
Same author

Proteomic profile of bone in patients with acromegaly: new insights from a pilot exploratory study.

Journal of endocrinological investigation·2026
Same author

Human Chorionic Gonadotropin (hCG)-Induced Remodeling of the Granulosa Cell Exosomal Proteome: Implications for Follicular Communication.

Cells·2026
Same author

Personalized automatic management of tracheal cuff pressure and subglottic secretions drainage to prevent pneumonia in critically ill intubated patients. The MICROINHALO multicenter randomized controlled trial.

Intensive care medicine·2026
Same author

Ultrasound-guided vs conventional venous puncture for atrial fibrillation ablation: the ULYSSES trial.

European heart journal·2026

Related Experiment Video

Updated: May 16, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Unraveling the different proteomic platforms.

Irene Messana1, Tiziana Cabras, Federica Iavarone

  • 1Dipartimento di Scienze della Vita e dell'Ambiente, Università di Cagliari, Cagliari, Italy.

Journal of Separation Science
|December 6, 2012
PubMed
Summary

This review introduces proteomics strategies for scientists outside the field, covering bottom-up and top-down platforms for qualitative and quantitative analysis. It explores data validation, post-translational modification (PTM) enrichment, and quantification methods for advancing functional proteomics and systems biology.

More Related Videos

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
07:38

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

Related Experiment Videos

Last Updated: May 16, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
07:38

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Systems Biology

Background:

  • Proteomics offers powerful strategies for biological investigation.
  • Scientists outside the field require an accessible overview of current proteomics techniques.

Purpose of the Study:

  • To provide an overview of modern proteomics strategies for non-specialists.
  • To critically examine qualitative and quantitative proteomics platforms.
  • To discuss the integration of proteomics in functional and systems biology research.

Main Methods:

  • Critical examination of bottom-up and top-down proteomics platforms.
  • Description of discovery, directed, and targeted proteomics approaches.
  • Analysis of enrichment methods for post-translational modifications (PTMs).
  • Comparison of label-free and labeled quantification techniques.

Main Results:

  • Outlined advantages and limitations of various proteomics platforms.
  • Defined different proteomics strategies for mass spectrometry (MS) management.
  • Discussed data validation and PTM elucidation methods.
  • Compared relative and absolute quantification approaches.

Conclusions:

  • Proteomics platforms are essential for advancing structural to functional proteomics.
  • Integration of proteomics is key for tackling complex systems biology challenges.
  • Careful experimental design and data validation are crucial for meaningful results.