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Related Experiment Video

Updated: May 16, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Targeting loop adenines in G-quadruplex by a selective oxirane.

Filippo Doria¹, Matteo Nadai², Marco Folini³

¹Dipartimento di Chimica, Università di Pavia, V.le Taramelli 10, 27100 Pavia (Italy).

Chemistry (Weinheim an Der Bergstrasse, Germany)

|December 6, 2012

Summary

Researchers synthesized naphthalene diimides as DNA G-quadruplex alkylating agents. An oxirane derivative selectively alkylates adenine in G-quadruplex loops, forming a stable adduct.

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Last Updated: May 16, 2026

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Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Area of Science:

Medicinal Chemistry
Organic Synthesis
Molecular Biology

Background:

DNA G-quadruplexes are non-canonical DNA structures implicated in various biological processes.
Targeting G-quadruplexes offers potential therapeutic strategies for diseases like cancer.
Developing selective agents for G-quadruplexes remains a challenge.

Purpose of the Study:

To synthesize and investigate naphthalene diimide derivatives as selective DNA G-quadruplex alkylating agents.
To evaluate the potential of an oxirane-conjugated naphthalene diimide for G-quadruplex modification.

Main Methods:

Synthesis of naphthalene diimides conjugated to quinone methide and oxirane moieties.
Investigation of alkylating activity against DNA G-quadruplex structures.
Characterization of adduct formation and selectivity using biophysical and biochemical techniques.

Main Results:

Successful synthesis of novel naphthalene diimide conjugates.
The oxirane derivative demonstrated selective alkylation of G-quadruplex DNA.
Stable adduct formation was observed, specifically targeting adenine residues within G-quadruplex loops.

Conclusions:

Naphthalene diimide-oxirane conjugates are promising selective DNA G-quadruplex alkylating agents.
The oxirane derivative's ability to form stable, selective adducts highlights its therapeutic potential.
Further studies are warranted to explore the in vivo efficacy and applications of these compounds.