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Upstream Processing

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Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

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Understanding drugs, drug products, and their performance in pharmaceutical science is pivotal. Drugs, whether simple molecules or complex compounds, are designed to interact with the body's biological systems to diagnose, treat, or prevent diseases. Drug products include various delivery systems such as tablets, capsules, injections, and inhalers. The performance of these drug products is gauged by their ability to deliver the active ingredient to the desired site of action at the appropriate...

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Drug Products: Biologics, Biosimilars and Interchangeables

Biologics, derived from living sources such as humans, animals, or microorganisms, represent a significant category of pharmaceuticals. These complex molecules, developed through advanced biotechnological methods or purified from natural sources, include essential medical treatments like insulin and growth hormones. The complexity of biologics arises from their large molecular structures and the intricate processes required for their production, making them distinct from conventional...

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Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...

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Updated: May 16, 2026

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Published on: September 22, 2011

Biopharmaceuticals -- discovery, development and manufacturing.

Alois Jungbauer, Klaus Graumann

Biotechnology Journal

|December 6, 2012

Summary

This special issue explores biopharmaceutical development, covering antigen presentation for molecule selection, G-protein-coupled receptors as drug targets, and advanced continuous downstream processing techniques.

Area of Science:

Biopharmaceutical research and development
Drug discovery and manufacturing
Molecular biology and pharmacology

Background:

This special issue compiles research on key stages in biopharmaceutical development.
It addresses critical aspects from initial discovery to large-scale manufacturing.

Discussion:

Examines the role of antigen presentation in the creation and selection of effective binding molecules.
Highlights G-protein-coupled receptors (GPCRs) as crucial targets for novel therapeutic interventions.
Discusses the implementation of continuous downstream processing for enhanced manufacturing efficiency.

Key Insights:

Understanding antigen presentation is vital for designing targeted biotherapeutics.
GPCRs represent a significant area for drug development.

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Last Updated: May 16, 2026

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Published on: September 22, 2011

Laboratory Scale Production and Purification of a Therapeutic Antibody

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

Continuous processing offers a pathway to more efficient biopharmaceutical manufacturing.

Outlook:

Future research directions in biopharmaceutical discovery and engineering.
Advancements in manufacturing technologies for biologics.
The evolving landscape of therapeutic protein development.