Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Complementation with ts mutants of herpes simplex virus.

L I Messer, M C Timbury

    The Journal of General Virology
    |November 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A favourite score.

    British medical journal·2010
    Same author

    Influenza types and patient population.

    Lancet (London, England)·1995
    Same author

    Non-polio enteroviruses and motor neurone disease.

    Journal of clinical pathology·1988
    Same author

    How to deal with a hospital outbreak of Legionnaires' disease.

    The Journal of hospital infection·1988
    Same author

    Use of ev loci as a measure of inbreeding in domestic fowls.

    British poultry science·1987
    Same author

    Rapid diagnosis of an outbreak of Legionnaires' disease at Glasgow Royal Infirmary.

    Thorax·1987
    Same journal

    Variability and population structure of watermelon mosaic virus in zucchini crops in Poland.

    The Journal of general virology·2026
    Same journal

    Probing the sequence constraints for the stable incorporation of chimeric NSP1 segments into infectious rotaviruses.

    The Journal of general virology·2026
    Same journal

    Assessment of influenza virus and coronavirus tropism, replication competence and disease severity in <i>ex vivo</i> and <i>in vitro</i> cultures of the human respiratory tract.

    The Journal of general virology·2026
    Same journal

    GRHAL1, a novel lncRNA, regulates HIV-1 gene expression by modulating Tat- and Sp1-mediated HIV-1 LTR activation.

    The Journal of general virology·2026
    Same journal

    Hepatitis B virus X protein induces E6-associated protein-mediated proteasomal degradation of p53 phosphorylated at Ser-15.

    The Journal of general virology·2026
    Same journal

    Sensing the storm: how inflammatory signalling drives reactivation of the human cytomegalovirus major immediate-early promoter.

    The Journal of general virology·2026
    See all related articles

    Herpes simplex virus complementation tests reveal that non-infectious viral particles can contribute to the process. This research explored genetic recombination and virus DNA synthesis in mutant herpes simplex virus types.

    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Background:

    • Herpes simplex virus (HSV) exhibits genetic diversity, with distinct types 1 and 2.
    • Temperature-sensitive (ts) mutants are valuable tools for studying viral replication and genetic interactions.
    • Complementation analysis helps understand genetic relationships between viral mutants.

    Purpose of the Study:

    • To investigate complementation between ts mutants of herpes simplex virus types 1 and 2.
    • To analyze progeny virus production and genetic recombination in intratypic and intertypic complementation assays.
    • To explore the role of non-infectious particles in viral complementation.

    Main Methods:

    • Infectious centre and virus yield assays were employed to detect complementation.

    Related Experiment Videos

  • Progeny analysis was conducted for both intratypic (within the same type) and intertypic (between types 1 and 2) complementation.
  • Virus DNA synthesis was monitored in mixedly-infected cells at non-permissive temperatures to detect complementation between DNA-negative mutants.

    • Main Results:

    • A significant proportion of recombinant or wild-type (ts+) virus was observed in the progeny of both intratypic and intertypic complementation tests.
    • Complementation occurred even at very low multiplicities of infection, suggesting a role for non-infectious viral particles.
    • Intermediate temperature-sensitive virus was produced during both types of complementation.
    • Complementation between DNA-negative mutants was successfully detected by monitoring viral DNA synthesis.

    Conclusions:

    • Non-infectious herpes simplex virus particles likely play a role in the complementation process.
    • Complementation assays, including those using DNA-negative mutants, are effective for studying genetic interactions in HSV.
    • The study provides insights into the genetic complexity and recombination potential of herpes simplex virus types 1 and 2.