Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of Cereblon-Directing Warheads for the Development of Orally Bioavailable PROTACs.

Journal of medicinal chemistry·2025
Same author

Leveraging Dual-Ligase Recruitment to Enhance Protein Degradation via a Heterotrivalent Proteolysis Targeting Chimera.

Journal of the American Chemical Society·2024
Same author

Structure-Activity Relationship of Potent, Selective, and Orally Bioavailable Molecular Glue Degraders of CK1α.

ACS medicinal chemistry letters·2024
Same author

Addressing common challenges of biotherapeutic protein peptide mapping using recombinant trypsin.

Journal of pharmaceutical and biomedical analysis·2024
Same author

Selective CK1α degraders exert antiproliferative activity against a broad range of human cancer cell lines.

Nature communications·2024
Same author

Monitoring PROTAC interactions in biochemical assays using Lumit immunoassays.

Methods in enzymology·2023
Same journal

A homogenous luminescence assay reveals novel inhibitors for giardia lamblia carbamate kinase.

Current chemical genomics·2013
Same journal

A comparison of the performance and application differences between manual and automated patch-clamp techniques.

Current chemical genomics·2013
Same journal

High-Throughput Multiplexed Quantitation of Protein Aggregation and Cytotoxicity in a Huntington's Disease Model.

Current chemical genomics·2013
Same journal

Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands.

Current chemical genomics·2012
Same journal

A Dual Reporter Splicing Assay Using HaloTag-containing Proteins.

Current chemical genomics·2012
Same journal

In Vivo Fluorescent Labeling of Tumor Cells with the HaloTag® Technology.

Current chemical genomics·2012
See all related articles

Related Experiment Video

Updated: May 16, 2026

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
11:16

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

Published on: July 12, 2014

HaloTag, a Platform Technology for Protein Analysis.

Marjeta Urh1, Martin Rosenberg

  • 1Promega Corporation, Madison, WI, USA.

Current Chemical Genomics
|December 6, 2012
PubMed
Summary
This summary is machine-generated.

The HaloTag platform offers a versatile technology for studying protein interactions and functions. This protein fusion tool enables comprehensive analysis of proteomic biology across various biological systems.

Keywords:
HaloTag technologyprotein fusion tagsprotein imagingprotein interactionsprotein purification.

More Related Videos

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

Related Experiment Videos

Last Updated: May 16, 2026

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
11:16

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

Published on: July 12, 2014

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

Area of Science:

  • Proteomics
  • Molecular Biology
  • Biochemistry

Background:

  • Protein interactions are crucial for biological processes and disease.
  • Understanding complex protein networks requires advanced analytical tools.
  • Existing methods may not fully capture the dynamic nature of protein interactions.

Purpose of the Study:

  • To introduce and review the HaloTag protein fusion technology.
  • To highlight the platform's utility in studying protein biology.
  • To demonstrate its applications in various biological contexts.

Main Methods:

  • Development and application of the HaloTag protein fusion technology.
  • In vitro and in vivo performance evaluation.
  • Utilizing HaloTag for protein imaging, isolation, and complex analysis.

Main Results:

  • HaloTag provides a powerful tool for comprehensive proteomic analysis.
  • The technology demonstrates multifunctional utility across diverse applications.
  • Successful application in biochemical, cell-based, and whole-animal studies.

Conclusions:

  • The HaloTag platform significantly advances the study of protein function and interactions.
  • It offers a robust solution for global analyses of proteomic events.
  • Enables deeper understanding of complex biological systems at multiple levels.