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Updated: May 16, 2026

Preparation of Zinc Oxide Nanoparticles and the Evaluation of their Antibacterial Effects

Preparation of Zinc Oxide Nanoparticles and the Evaluation of their Antibacterial Effects

Published on: September 27, 2024

Paper modified with ZnO nanorods - antimicrobial studies.

Mayuree Jaisai¹, Sunandan Baruah, Joydeep Dutta

¹Center of Excellence in Nanotechnology, Asian Institute of Technology, Klong Luang, Pathumthani 12120, Thailand.

Beilstein Journal of Nanotechnology

|December 6, 2012

Summary

Antimicrobial paper with zinc oxide nanorods effectively inhibits bacteria and fungi. This innovative material shows significant potential for hygiene applications in healthcare settings.

Keywords:

antimicrobial nanorod paper photocatalysis zinc oxide

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Area of Science:

Materials Science
Microbiology
Nanotechnology

Background:

Antimicrobial materials are crucial for preventing infections in healthcare.
Developing novel antimicrobial surfaces is an ongoing research area.
Zinc oxide nanorods (ZnO NRs) exhibit promising antimicrobial properties.

Purpose of the Study:

To develop antimicrobial paper using in situ growth of ZnO nanorods.
To evaluate the efficacy of the developed paper against common microbes.
To assess the influence of lighting conditions on antimicrobial activity.

Main Methods:

In situ growth of ZnO nanorods on paper substrates.
Antimicrobial testing against Staphylococcus aureus (Gram-positive), Escherichia coli (Gram-negative), and Aspergillus niger (fungus).
Zone of inhibition assays under halogen and fluorescent lighting.

Main Results:

Complete inhibition of viable bacterial colonies and fungal spores around the antimicrobial paper samples.
Significant zones of inhibition observed for E. coli (239% and 163%), S. aureus (102% and 70%), and A. niger (224% and 183%) under different lighting conditions.
Antimicrobial activity was confirmed, with varying efficacy depending on the microbe and illumination.

Conclusions:

The developed ZnO nanorod-based paper possesses potent antimicrobial properties.
This material is a promising candidate for applications in healthcare environments, such as wallpaper and facemasks.
Further research can optimize ZnO nanorod integration for enhanced antimicrobial performance.