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Updated: May 16, 2026

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
Published on: September 17, 2016
Self-arrangement of cellular circadian rhythms through phase-resetting in plant roots
Hirokazu Fukuda1, Kazuya Ukai, Tokitaka Oyama
1Department of Mechanical Engineering, Graduate School of Engineering, Osaka Prefecture University, Sakai 599-8531, Japan. fukuda@me.osakafu-u.ac.jp
Abstract:
We discovered a striped pattern of gene expression with circadian rhythms in growing plant roots using bioluminescent imaging of gene expression. Our experimental analysis revealed that the stripe wave in the bioluminescent image originated at the root tip and was caused by a continuous phase resetting of circadian oscillations. Some complex stripe waves containing arrhythmic regions were also observed. We succeeded in describing the formation mechanisms of these patterns using a growing phase oscillator network with a phase-resetting boundary condition.
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