Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fibulin-4 expressed in metastatic breast cancer is a target of peptide-based imaging probes and experimental therapeutics.

Molecular therapy. Oncology·2026
Same author

Turep: Detecting cross-cancer tumor-reactive T cells in single-cell and spatial transcriptomics data.

bioRxiv : the preprint server for biology·2026
Same author

Investigating machine learning algorithms to classify label-free images of pancreatic neuroendocrine neoplasms.

Biophotonics discovery·2026
Same author

Telomerase Knockout in Myeloid Cells Predisposes Mice to Foam Cell Formation, Dyslipidemia, Lung Fibrosis, and Cardiac Dysfunction.

Aging cell·2026
Same author

Lymphatic pumping failure in the arm precedes dermal backflow and breast cancer-related lymphedema.

Breast cancer research : BCR·2026
Same author

Molecularly Engineered Memristors for Reconfigurable Neuromorphic Functionalities.

Advanced materials (Deerfield Beach, Fla.)·2025

Related Experiment Video

Updated: May 16, 2026

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
10:55

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

Multimodal chelation platform for near-infrared fluorescence/nuclear imaging.

Sukhen C Ghosh1, Pradip Ghosh, Nathaniel Wilganowski

  • 1Center for Molecular Imaging, The Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston , Houston, Texas 77030, USA.

Journal of Medicinal Chemistry
|December 11, 2012
PubMed
Summary

A novel multimodality chelation (MMC) scaffold simplifies dual labeling for cancer imaging. This approach enables enhanced tumor visualization for surgical guidance using nuclear and near-infrared fluorescence contrast agents.

More Related Videos

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Multimodal Cross-Device and Marker-Free Co-Registration of Preclinical Imaging Modalities
07:13

Multimodal Cross-Device and Marker-Free Co-Registration of Preclinical Imaging Modalities

Published on: October 27, 2023

Related Experiment Videos

Last Updated: May 16, 2026

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
10:55

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Multimodal Cross-Device and Marker-Free Co-Registration of Preclinical Imaging Modalities
07:13

Multimodal Cross-Device and Marker-Free Co-Registration of Preclinical Imaging Modalities

Published on: October 27, 2023

Area of Science:

  • Biomedical imaging
  • Molecular imaging
  • Cancer diagnostics

Background:

  • Dual-labeled compounds combine nuclear and near-infrared fluorescence for surgical cancer resection guidance.
  • Current dual labeling processes for antibody-based agents are complex.
  • A simplified approach is needed to integrate whole-body imaging with surgical visualization.

Purpose of the Study:

  • To develop a multimodality chelation (MMC) scaffold for simplified dual labeling.
  • To create antibody-based agents with combined radiometal and fluorescence imaging capabilities.
  • To assess the efficacy of the MMC scaffold in a prostate cancer model.

Main Methods:

  • Synthesized three dye-derivatized MMC compounds and radiolabeled them.
  • Prepared an EpCAM-targeting MMC-immunoconjugate dual-labeled with 64Cu.
  • Evaluated in vitro binding activity and in vivo tumor accumulation using multimodal imaging.

Main Results:

  • IRDye 800CW conjugate (4) exhibited favorable optical properties and rapid in vivo clearance.
  • Confirmed in vitro binding activity of the MMC-conjugated immunoconjugate.
  • Demonstrated higher tumor accumulation of 64Cu-7 compared to nontargeted 64Cu-4 in a prostate cancer model.

Conclusions:

  • The MMC scaffold simplifies dual labeling for antibody-based imaging agents.
  • Dual-labeled agents show potential for improved tumor targeting and visualization.
  • Further evaluation in diverse EpCAM-expressing cell lines and with other antibodies is recommended.