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Applications for Open Source Microplate-Compatible Illumination Panels

Applications for Open Source Microplate-Compatible Illumination Panels

Published on: October 3, 2019

Surface-scribed transparency-based microplates.

Xin Ye Li¹, Brandon Huey-Ping Cheong, Anthony Somers

¹Laboratory for Optics, Acoustics, & Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria 3800, Australia.

Langmuir : the ACS Journal of Surfaces and Colloids

|December 11, 2012

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Scribed transparency microplates offer a low-cost optical diagnostic method. These novel microplates demonstrate improved liquid handling and retention, without compromising fluorescence measurement performance.

Area of Science:

Materials Science
Analytical Chemistry
Biotechnology

Background:

Transparency sheets offer a cost-effective substrate for microplate instrumentation.
Traditional microplates can be expensive, limiting widespread use in certain diagnostic applications.

Purpose of the Study:

To investigate a novel microplate design using scribed transparency sheets for optical diagnostics.
To evaluate the liquid handling properties, including contact angle and volume retention, of these new microplates.
To assess the impact of the scribed design on fluorescence measurement performance.

Main Methods:

Optical profilometry to characterize the 3D profile of scribed regions.
Contact angle measurements during liquid droplet dispensation.

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Volume retention and tilt angle tests to determine liquid displacement thresholds.

Fluorescence measurements comparing scribed transparency microplates with standard microplates.

Main Results:

Scribed regions significantly influenced three-phase contact line interactions and contact angles.
The microplates demonstrated a full well volume of 50 μL and a maximum retainable volume of 190 μL.
Reduced tilt angles were required for liquid displacement with scribed surfaces compared to non-scribed ones.
Dispensing liquid at the center improved well filling efficiency.
Fluorescence measurements showed no performance compromise compared to standard microplates.

Conclusions:

Scribed transparency microplates provide a viable, low-cost alternative for microplate instrumentation.
The design enhances liquid handling capabilities, offering better control over volume retention and displacement.
The proposed microplates maintain optical diagnostic performance, making them suitable for various applications.