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Published on: April 11, 2016
Multi-fingerprint and quality control analysis of tea polysaccharides
Yuanfeng Wang1, Jianghui Xian, Xionggang Xi
1Institute of Food Engineering, College of Life & Environment Science, Shanghai Normal University, 100 Guilin Rd, Shanghai 200234, PR China.
Carbohydrate Polymers
|December 11, 2012
Summary
Multi-fingerprint analysis using chromatography and spectroscopy confirms the quality control of tea polysaccharides (TPS). This method effectively distinguishes TPS from other polysaccharides, ensuring product consistency and accuracy.
Area of Science:
- Analytical Chemistry
- Food Science
- Pharmacognosy
Background:
- Tea polysaccharides (TPS) are complex natural products with significant biological activities.
- Ensuring the quality and consistency of TPS is crucial for their reliable application.
- Traditional quality control methods may not fully capture the complexity of TPS.
Purpose of the Study:
- To develop and validate a multi-fingerprint analytical method for tea polysaccharide (TPS) quality control.
- To assess the consistency of different batches of TPS.
- To differentiate TPS from other polysaccharide substances.
Main Methods:
- Utilized High-Performance Gel Permeation Chromatography (HPGPC) for molecular size analysis.
- Employed Ultraviolet-Visible (UV) and Infrared Absorption (IR) spectroscopy for chemical structure elucidation.
- Applied Ion Chromatography (IC) for ionic component analysis.
- Calculated similarity using angle cosine and correlation coefficient methods on spectral and chromatographic data.
Main Results:
- High similarity (up to 0.9985 and 0.9475) was observed among the UV, IR, and IC fingerprints of 22 TPS batches.
- Distinct differences were identified when comparing TPS fingerprints with five other polysaccharides.
- The multi-fingerprint approach proved more effective than single fingerprint methods for TPS quality assessment.
Conclusions:
- The developed multi-fingerprint method is rapid, effective, visual, and accurate for TPS research and quality control.
- This approach reliably confirms the consistency of TPS batches.
- The method successfully distinguishes TPS from other polysaccharides, highlighting its utility in quality assurance.
