Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multiplexed single-tier immunoassay for improved Lyme disease diagnosis across all disease stages.

Research square·2026

Same author

<i>BRAF</i> V600E Mutation Has Variable Tumor-Specific Effects on Expression of MAPK Pathway Genes That Could Affect Patient Outcome.

International journal of molecular sciences·2025

Same author

High-sensitivity multicancer detection of stage 1 cancer in dogs.

American journal of veterinary research·2025

Same author

Freezing diluted bovine serum albumin standards does not significantly affect standard curves.

BioTechniques·2025

Same author

Profiling muscle transcriptome in mice exposed to microgravity using gene set enrichment analysis.

NPJ microgravity·2024

Same author

A Peptide Microarray Platform Approach for Discovery of Immunodominant Antibody Epitopes.

Analytical chemistry·2024

Search research articles

Related Experiment Video

Updated: May 16, 2026

Immunoblot Analysis

Immunoblot Analysis

Published on: June 20, 2008

Immunosignaturing: a critical review.

Kathryn F Sykes¹, Joseph B Legutki, Phillip Stafford

¹HealthTell Inc., 145 South 79th Street, Chandler, AZ 85226, USA.

Trends in Biotechnology

|December 11, 2012

Summary

This summary is machine-generated.

Immunosignaturing, a microarray technique, quantifies circulating antibodies to assess health changes. This review explores its advancements, applications, and compares it to similar immune monitoring technologies.

More Related Videos

Imaging the Human Immunological Synapse

Imaging the Human Immunological Synapse

Published on: December 26, 2019

Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis

Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis

Published on: September 26, 2012

Related Experiment Videos

Last Updated: May 16, 2026

Immunoblot Analysis

Immunoblot Analysis

Published on: June 20, 2008

Imaging the Human Immunological Synapse

Imaging the Human Immunological Synapse

Published on: December 26, 2019

Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis

Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis

Published on: September 26, 2012

Area of Science:

* Biomedical science
* Immunology
* Biotechnology

Background:

* Health is a complex interplay of metabolic, physiological, and immune factors.
* The humoral immune system's activity serves as a quantifiable proxy for health status.
* Circulating antibody dynamics reflect immune system activity.

Purpose of the Study:

* To review recent advancements in immunosignaturing technology.
* To introduce the immunosignaturing technique and its principles.
* To explore potential applications and compare it with similar technologies.

Main Methods:

* Microarray-based immunosignaturing quantifies antibody profiles.
* Analysis of circulating antibody dynamics.
* Comparative evaluation of different immune monitoring technologies.

Main Results:

* Immunosignaturing offers a quantitative measure of immune system activity.
* Recent technological progress enhances the utility of immunosignaturing.
* The technique shows promise for various health-related applications.

Conclusions:

* Immunosignaturing is a valuable tool for assessing health via antibody profiling.
* Further development could expand its diagnostic and research capabilities.
* Comparison with other technologies highlights its unique advantages.