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Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
Published on: April 27, 2011
Surface-activated microtiter-plate microarray for simultaneous CRP quantification and viral antibody detection.
Sari M Viitala1, Anne J Jääskeläinen, Eira Kelo
1School of Pharmacy, University of Eastern Finland, Kuopio, Finland.
Diagnostic Microbiology and Infectious Disease
|December 11, 2012
Summary
This study developed a novel microarray method using standard 96-well plates for detecting antibodies and C-reactive protein (CRP). This platform shows promise for improved diagnostics in infectious diseases and autoimmune conditions.
Area of Science:
- Biotechnology
- Immunology
- Diagnostics
Background:
- Microarrays are essential tools for high-throughput biological assays, expanding into protein and small molecule studies.
- Parallel detection of antibodies and antigens offers significant potential in epidemiology, vaccine development, and diagnosing allergies, autoimmunity, and infectious diseases.
Purpose of the Study:
- To demonstrate an immobilization method for an immunoassay-based microarray on conventional 96-well polystyrene plates.
- To combine serologic diagnostic capabilities with quantitative C-reactive protein (CRP) assay.
Main Methods:
- Utilized a synthetic peptide (HIV-1), recombinant protein (Puumala hantavirus nucleocapsid), and whole virus preparations (Sindbis, adenoviruses) as antigens for antibody detection.
- Employed monoclonal anti-CRP antibody for antigen detection.
- Based the microarray on enzyme immunoassays with densitometry from photographed results.
Main Results:
- Peptide and recombinant antigens performed effectively; whole virus antigens showed minor discrepancies (1/23 samples) compared to a reference method.
- Quantitative C-reactive protein (CRP) assay results demonstrated high concordance with two commercial assays (R² = 0.9975 and R² = 0.9595) in the 0.5-150 mg/L range.
Conclusions:
- Microtiter plates serve as a viable and promising platform for developing advanced microarrays.
- This approach facilitates parallel antibody and antigen detection, advancing diagnostic capabilities.
