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Iron mobilization from asbestos by chelators and ascorbic acid.

L G Lund1, A E Aust

  • 1Department of Chemistry and Biochemistry, Utah State University, Logan 84322-0300.

Archives of Biochemistry and Biophysics
|April 1, 1990
PubMed
Summary

Low-molecular-weight chelators, including ascorbic acid, can mobilize iron from asbestos fibers. This iron mobilization may disrupt cellular iron metabolism and cause oxidative damage to biomolecules.

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Area of Science:

  • Environmental Science
  • Toxicology
  • Biochemistry

Background:

  • Asbestos exposure is linked to various health issues, including cancer.
  • Iron released from asbestos may play a role in asbestos-induced toxicity.
  • Understanding iron mobilization mechanisms is crucial for assessing asbestos-related health risks.

Purpose of the Study:

  • To investigate the ability of different chelators and ascorbic acid to mobilize iron from various asbestos types.
  • To determine the influence of chelator type, asbestos properties, and ascorbate on iron mobilization rates.
  • To assess the potential health implications of iron mobilization from asbestos within cellular environments.

Main Methods:

  • Incubation of asbestos fibers (crocidolite, amosite, chrysotile, tremolite) with chelators (ferrozine, citrate, ADP) and ascorbic acid in a buffered saline solution.

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  • Quantification of mobilized iron (Fe(II) and Fe(III)) using spectrophotometric methods.
  • Analysis of iron mobilization rates as a function of chelator concentration, asbestos type, and presence of ascorbate.

    • Main Results:

    • Ferrozine effectively mobilized Fe(II) from crocidolite and amosite, with rates significantly enhanced by ascorbate.
    • Ascorbate was necessary for ferrozine to mobilize Fe(II) from medium-fiber chrysotile.
    • Citrate and ADP mobilized iron from crocidolite and amosite, with citrate showing higher rates, also enhanced by ascorbate.
    • Iron mobilization was influenced by asbestos surface area, crystalline structure, and iron content.

    Conclusions:

    • Low-molecular-weight chelators can mobilize iron from asbestos fibers.
    • Ascorbic acid potentiates iron mobilization by chelators and can mobilize iron independently.
    • Mobilized asbestos iron could disrupt intracellular iron homeostasis, potentially leading to oxidative stress and cellular damage.