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The Ala16Val MnSOD gene polymorphism modulates oxidative response to exercise.
Guilherme Bresciani1, Javier González-Gallego, Ivana B da Cruz
1Institute of Biomedicine, University of León, Spain. guilhermebresciani@gmail.com.
Clinical Biochemistry
|December 11, 2012
Summary
The manganese-superoxide dismutase (MnSOD) Ala16Val gene polymorphism influences how the body responds to exercise-induced oxidative stress. The AA genotype enhances MnSOD activity post-exercise, unlike the VV genotype.
Area of Science:
- Human genetics
- Exercise physiology
- Biochemistry
Background:
- The manganese-superoxide dismutase (MnSOD) gene's Ala16Val polymorphism is linked to metabolic disorders.
- Environmental factors may influence the disease risks associated with this MnSOD polymorphism.
- Limited research exists on exercise-induced oxidative stress modulation by the MnSOD Ala16Val polymorphism.
Purpose of the Study:
- To investigate whether exercise-induced oxidative stress is differentially modulated by the MnSOD Ala16Val polymorphism.
- To explore the impact of MnSOD genotypes on cellular responses to acute exercise.
Main Methods:
- Genotyping of 57 males for the MnSOD Ala16Val polymorphism.
- Selection of 10 subjects per genotype (AA and VV) for controlled intense exercise.
- Measurement of MnSOD mRNA, protein, activity, glutathione, and thiol content in peripheral blood mononuclear cells before and after exercise.
Main Results:
- AA genotype: Increased post-exercise MnSOD mRNA expression and enzyme activity.
- VV genotype: No change in MnSOD mRNA, but decreased protein thiol content post-exercise.
- AA genotype showed higher MnSOD protein content than VV post-exercise; a dose-effect of the A allele on enzyme activity was observed.
Conclusions:
- MnSOD gene variants are associated with differential oxidative stress modulation during exercise.
- Environmental factors, such as exercise, may modify disease risks linked to the MnSOD Ala16Val polymorphism.
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