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[Subrenal capsule assay using nude mice].

H Kikuchi¹, M Asamura, M Gamoh

¹Dept. of Clinical Cancer Chemotherapy, Tohoku University.

Gan to Kagaku Ryoho. Cancer & Chemotherapy

|March 1, 1990

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The subrenal capsule assay (SRCA) in nude mice offers a more accurate in vivo cancer drug sensitivity test by minimizing host immune reactions. Adjusting assay duration and drug dosage is crucial for reliable chemosensitivity results.

Area of Science:

Oncology
Immunology
Pharmacology

Background:

The subrenal capsule assay (SRCA) is a valuable in vivo method for evaluating anticancer drug efficacy.
Host immune responses in immunocompetent mice can interfere with accurate chemosensitivity testing in SRCA.
Nude mice, lacking a functional immune system, may provide a more reliable model for SRCA.

Purpose of the Study:

To compare tumor growth kinetics and host reactions in normal immunocompetent versus nude mice using the SRCA.
To evaluate the impact of immunosuppressive drugs, cyclophosphamide (EX) and cyclosporin A (CSA), on SRCA outcomes.
To determine optimal conditions for SRCA, considering host factors and drug toxicities.

Main Methods:

Comparison of tumor growth and histological findings in normal and nude mice within the SRCA.

Related Experiment Videos

Assessment of tumor response to cyclophosphamide (EX) and cyclosporin A (CSA) in SRCA.

Evaluation of adriamycin (ADR) chemosensitivity in nude mice and the influence of CSA on drug toxicity.

Main Results:

Nude mice exhibited prolonged tumor growth (16 days) with preserved tumor cells compared to normal mice (6 days) with significant host reactions.
EX and CSA treatments altered tumor growth dynamics, with CSA-treated tumors showing better preservation.
Adriamycin demonstrated efficacy in nude mice, but concurrent CSA administration necessitated dose adjustments to avoid toxicity, potentially leading to false negatives.

Conclusions:

Nude mice are superior to immunocompetent mice for SRCA due to reduced host immune interference.
A 12-day SRCA is recommended for nude mice, while a 6-day assay may be suitable for EX-treated normal mice.
Cyclosporin A's potential antitumor effects and its influence on drug toxicity require careful consideration in SRCA protocols.