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Updated: May 16, 2026
13:10
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
Published on: June 9, 2017
Tools and strategies for DNA damage interactome analysis
Christophe Bounaix Morand du Puch1, Ewa Barbier1, Sylvie Sauvaigo1
1Laboratoire Lésions des Acides Nucléiques, SCIB, UMR-E3 CEA/UJF-Grenoble 1, INAC, 17 rue des Martyrs, Grenoble, F-38054, France.
Mutation Research
|December 11, 2012
Summary
Proteomics methods identify new proteins interacting with DNA damage, aiding understanding of cellular responses to genotoxic agents and anticancer drugs. These findings may reveal new biomarkers and therapeutic targets.
Area of Science:
- Molecular Biology
- Proteomics
- Genetics
Background:
- DNA is constantly exposed to damaging agents, triggering cellular responses involving numerous proteins.
- The DNA damage interactome includes proteins involved in chromatin remodeling, DNA repair, and transcription.
- Proteomics approaches have become crucial for mapping these complex protein networks.
Purpose of the Study:
- To explore and characterize the protein interactome associated with DNA damage.
- To identify novel proteins that recognize various types of DNA lesions.
- To investigate protein interactions induced by genotoxic stress and anticancer drugs.
Main Methods:
- Utilizing proteomics-based strategies to identify DNA-interacting proteins.
- Employing damaged DNA molecules as targets in protein microarrays.
- Using damaged DNA probes to capture and identify proteins from cellular extracts.
- Analyzing chromatin-bound proteins after genotoxic stress.
- Identifying proteins associated with known DNA damage response proteins.
Main Results:
- Several proteomics strategies have been successfully applied to discover new proteins interacting with DNA damage.
- These methods identified proteins recognizing oxidized bases, abasic sites, strand breaks, and crosslinks.
- Confirmed interactions include cisplatin-DNA crosslinks with PARP-1 and the PTW/PP complex.
- Complementary methods like electromobility shift assays and surface plasmon resonance validate identified interactions.
Conclusions:
- Proteomics has significantly advanced the characterization of the DNA damage interactome.
- Newly identified protein interactions offer insights into genetic toxicology and cellular responses to anticancer agents.
- Further research into these interactions could lead to the discovery of new biomarkers and therapeutic targets.
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