Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 16, 2026

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging
13:13

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

Published on: July 9, 2008

Insights into dendritic cell function using advanced imaging modalities.

Jatin M Vyas1

  • 1Division of Infectious Disease, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA. jvyas@partners.org

Virulence
|December 11, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Mission to Margin in Academic Medicine - The Impact of Corporate Medicine on Medical Training.

The New England journal of medicine·2026
Same author

Stabilizing and strengthening the US physician-scientist faculty workforce in academic medicine: a proposed institutional framework.

JCI insight·2026
Same author

Correction: Extracellular vesicles from diverse fungal pathogens induce species-specific and endocytosis-dependent immunomodulation.

PLoS pathogens·2026
Same author

Immunomodulatory functions of fungal melanins in respiratory infections.

mBio·2025
Same author

Single-cell analysis of human airway epithelium identifies cell-type-specific responses to <i>Aspergillus</i> and <i>Coccidioides</i>.

mBio·2025
Same author

Cross-Neutralization of Distant Coronaviruses Strongly Correlates with Spike S2-Specific Antibodies from Immunocompetent and Immunocompromised Vaccinated SARS-CoV-2-Infected Patients.

Vaccines·2025
Same journal

Piperine reverses colistin resistance in multidrug resistant Gram-negative pathogens by membrane disruption and ROS damage.

Virulence·2026
Same journal

Bridging environmental pollution and immune dysregulation: How microplastics disrupt gut homeostasis to promote allergic diseases.

Virulence·2026
Same journal

Genomic epidemiology, evolution, and transmission dynamics of porcine sapelovirus associated with diarrheic piglets.

Virulence·2026
Same journal

Hijacking and subversion of macrophage antiviral functions by viruses.

Virulence·2026
Same journal

Virulence attenuation in ST11-K64 <i>Klebsiella pneumoniae</i> explains its divergent clinical manifestation from ST23-K1.

Virulence·2026
Same journal

Nucleotide variations in 3' untranslated region contribute to the enhanced virulence and replication efficiency of reemerging Japanese encephalitis virus genotype V.

Virulence·2026
See all related articles

Advanced imaging reveals dendritic cell dynamics, challenging old ideas. Live cell microscopy shows how antigen-presenting cells and T cells interact in real-time, advancing immunology.

Area of Science:

  • Immunology
  • Cell Biology
  • Microscopy

Background:

  • Dendritic cell biology has historically relied on static imaging, leading to established but potentially incomplete understandings.
  • The evolution of imaging technologies has been crucial in advancing the field of immunology.
  • Live cell imaging in situ offers dynamic perspectives previously unattainable.

Purpose of the Study:

  • To review advanced microscopy techniques applicable to dendritic cell research.
  • To highlight how these techniques provide novel insights into dendritic cell function.
  • To demonstrate the importance of spatiotemporal dynamics in cell-cell interactions.

Main Methods:

  • Two-photon microscopy
  • Total internal reflection fluorescence (TIRF) microscopy
Keywords:
TIRFmicroscopyoptical trapsoptical tweezerspinning disktwo photon microscopy

More Related Videos

Imaging the Human Immunological Synapse
09:37

Imaging the Human Immunological Synapse

Published on: December 26, 2019

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis
11:28

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Published on: March 25, 2016

Related Experiment Videos

Last Updated: May 16, 2026

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging
13:13

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

Published on: July 9, 2008

Imaging the Human Immunological Synapse
09:37

Imaging the Human Immunological Synapse

Published on: December 26, 2019

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis
11:28

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Published on: March 25, 2016

  • Spinning disk confocal microscopy
  • Optical trapping

    • Main Results:

    • Live imaging challenges previous dogma in dendritic cell biology.
    • Spatiotemporal relationships between antigen-presenting cells and T cells are illuminated.
    • Dynamic visualization provides unprecedented insights into cellular interactions.

    Conclusions:

    • Advanced imaging techniques are revolutionizing dendritic cell research.
    • Live, in situ imaging is essential for understanding dynamic immunological processes.
    • The integration of advanced microscopy is key to future discoveries in immunology.