Cloning, heterologous expression, and enzymatic characterization of cathepsin L from starfish (Asterina pectinifera)
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Published on: November 23, 2016
A Ram Lee1, Hye Jin Bak, Na Young Kim
1Department of Biotechnology, Pukyong National University, Busan, Korea.
Bioscience, Biotechnology, and Biochemistry
|December 11, 2012
Abstract:
To determine the role of cathepsin L in Echinoderms, starfish (Asterina pectinifera) cathepsin L (ApCtL) was cloned. The results of RT-PCR analysis indicated that the expression of ApCtL in all of the tissues. The pro-mature enzyme of ApCtL, proApCtL, was expressed in Escherichia coli, and cathepsin activity was detected by cleaving of synthetic fluorogenic peptide substrates and gelatin zymography.
