Toxoflavin lyase enzyme as a marker for selecting potato plant transformants
Mi-Sun Kim1, HyunSoon Kim, Jae Sun Moon
1Green-Bio Research Center, KRIBB, Daejeon, Korea.
Bioscience, Biotechnology, and Biochemistry
|December 11, 2012
Abstract:
This study established a new system for potato transformation using toxoflavin as selection agent and toxoflavin lyase (tflA) as selectable marker gene. Potato plants expressing tflA was successfully transformed on toxoflavin medium with 27% efficiency, similar to that for the hygromycin/hpt selection system. The transgenic potato expressing tflA also showed resistance to Burkholderia glumea infection.


