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Related Experiment Video

Updated: May 16, 2026

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
08:35

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets

Published on: May 26, 2009

An effective purification method using large bottles for human pancreatic islet isolation.

Masayuki Shimoda1, Takeshi Itoh, Shuichi Iwahashi

  • 1Baylor University Medical Center, Dallas, TX, USA.unimogura@hotmail.co.jp

Islets
|December 11, 2012
PubMed
Summary

The top-loading bottle method significantly improves human islet purification, enhancing islet yield, recovery, and viability compared to the standard COBE method. This technique reduces mechanical stress, leading to better quality islets for transplantation.

Keywords:
bottle purificationislet isolationislet purificationislet transplantationshearing stress

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Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
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Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue

Published on: May 26, 2009

Area of Science:

  • Cell Biology
  • Transplantation Science
  • Biomedical Engineering

Background:

  • Pancreatic islet isolation and purification are critical for islet transplantation.
  • The standard COBE 2991 cell processor method causes mechanical damage to islets due to high shear forces.
  • Previous studies suggest bottle purification methods improve porcine islet purification.

Purpose of the Study:

  • To evaluate the efficacy of a novel bottle purification method for human islets.
  • To compare the top-loading and bottom-loading bottle methods against the standard COBE method.
  • To assess the impact of the bottle method on islet yield, purity, recovery, and viability.

Main Methods:

  • Human pancreata (n=9) from brain-dead donors were digested.
  • Digested tissue was divided into three purification groups: standard COBE, top-loading bottle, and bottom-loading bottle.
  • Continuous density gradients using ET-Kyoto and iodixanol solutions were employed.
  • Islet yield (IE/g), purity, recovery rate, and in vitro/in vivo viability were analyzed.

Main Results:

  • The top-loading bottle method yielded significantly higher islet yield (IE/g) and recovery rates than COBE and bottom-loading methods.
  • Purified islets in the top-loading group were larger, indicating reduced shear force.
  • In vivo viability was significantly improved with the top-loading bottle method compared to the COBE method.

Conclusions:

  • The top-loading bottle purification method enhances both the quantity and quality of human islets.
  • This method offers a less traumatic alternative to the standard COBE processor for islet purification.
  • Improved islet quality and quantity suggest better outcomes for islet transplantation.