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Turkey heterophil chemotaxis to Pasteurella multocida (serotype 3,4)-generated chemotactic factors
K S Latimer1, B G Harmon, J R Glisson
1Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Abstract:
The purpose of this study was to determine the ability of three strains or isolates of Pasteurella multocida (serotype 3,4) to generate chemotactic factors for heterophils when exposed to pooled turkey serum. Results indicated that each bacterial strain or isolate (M-9, CU, and 86-1913) was associated with the production of chemotactic factors, but the more pathogenic bacterial isolate (86-1913) elicited greater heterophil migration in chemotaxis studies.
Insights
Three Pasteurella multocida strains generated chemotactic factors for heterophils. The most pathogenic isolate, 86-1913, induced greater heterophil migration, indicating its role in avian inflammatory responses.
Area of Science:
- Veterinary Microbiology
- Immunology
- Poultry Disease Research
Background:
- Pasteurella multocida is a significant avian pathogen.
- Heterophils are key immune cells in poultry, crucial for combating bacterial infections.
- Understanding bacterial factors that influence heterophil activity is vital for disease control.
Purpose of the Study:
- To investigate the capacity of three Pasteurella multocida isolates (serotype 3,4) to produce chemotactic factors.
- To assess the effect of these factors on heterophil migration in turkeys.
- To compare the chemotactic potential among different P. multocida strains.
Main Methods:
- Exposure of pooled turkey serum to three Pasteurella multocida isolates (M-9, CU, 86-1913).
- Chemotaxis assays to measure heterophil migration.
- Evaluation of bacterial isolate pathogenicity.
Main Results:
- All three tested Pasteurella multocida isolates (M-9, CU, 86-1913) demonstrated the ability to generate chemotactic factors.
- The isolate designated 86-1913, identified as more pathogenic, induced a significantly greater migration of heterophils.
- Chemotactic factor production varied among the isolates, correlating with pathogenicity.
Conclusions:
- Pasteurella multocida strains, including serotype 3,4, actively produce factors that attract turkey heterophils.
- Higher pathogenicity in P. multocida isolates is associated with enhanced chemotactic factor production.
- These findings contribute to understanding the pathogenesis of P. multocida infections in poultry.