Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitosis trumps T stage and proposed international association for the study of lung cancer/american thoracic society/european respiratory society classification for prognostic value in resected stage 1 lung adenocarcinoma.

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancerยท2014
See all related articles

Related Experiment Video

Updated: May 16, 2026

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Tissue microarray technology and findings for diagnostic immunohistochemistry.

Anthony van Zwieten1

  • 1Department of Anatomical Pathology, Pathology Queensland-The Prince Charles Hospital, Chermside, Queensland, Australia. anthony_van_Zwieten@health.qld.gov.au

Pathology
|December 11, 2012
PubMed
Summary

Tissue microarray (TMA) technology is valid for diagnostic immunohistochemistry (IHC) quality control. This method effectively serves as a universal positive control, aiding pathologists and scientists in IHC staining interpretation.

More Related Videos

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment
06:32

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment

Published on: August 18, 2023

Related Experiment Videos

Last Updated: May 16, 2026

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment
06:32

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment

Published on: August 18, 2023

Area of Science:

  • Histopathology
  • Diagnostic Pathology
  • Biotechnology

Background:

  • Immunohistochemistry (IHC) is crucial for accurate cancer diagnosis.
  • Standardization and quality control in IHC are essential for reliable results.
  • Tissue microarray (TMA) technology offers a method for high-throughput tissue analysis.

Purpose of the Study:

  • To evaluate the validity and viability of tissue microarray (TMA) technology for diagnostic immunohistochemistry (IHC).
  • To assess TMA as a quality control tool in a single laboratory setting.

Main Methods:

  • Performed IHC using 57 primary antibodies on a TMA block with 89 cores of duplicate tissue specimens.
  • Interpreted IHC staining by a histology scientist, with pathologist consultation.
  • Conducted a literature review for unexpected immunoreactivity.

Main Results:

  • 55 of 57 antibodies showed expected positive staining, correlating with original paraffin blocks.
  • Duplicate cores on the TMA demonstrated 98% correlation with original tissue blocks.
  • Observed some instances of unexpected positive immunoreactivity.

Conclusions:

  • Tissue microarray (TMA) technology is effective for diagnostic IHC laboratories.
  • TMA can function as a universal positive multiple tissue control block for routine IHC.
  • TMA provides valuable data for improving IHC staining interpretation by scientists and pathologists.