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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: May 16, 2026

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
09:41

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Published on: April 1, 2014

Isolating lipid droplets from multiple species.

Yunfeng Ding1, Shuyan Zhang, Li Yang

  • 1National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Nature Protocols
|December 11, 2012
PubMed
Summary
This summary is machine-generated.

Researchers developed a simple, 7-hour protocol to isolate high-quality lipid droplets (LDs) from diverse tissues and organisms. This method supports broader research into LDs, crucial for metabolic syndromes and biofuel development.

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Last Updated: May 16, 2026

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
09:41

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Published on: April 1, 2014

Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit
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Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

Area of Science:

  • Cell biology
  • Biochemistry
  • Biotechnology

Background:

  • Lipid droplets (LDs) are vital organelles implicated in metabolic disorders and biofuel applications.
  • Current LD isolation methods require extensive optimization and are not universally applicable.
  • Expanding LD research to new model organisms and tissues necessitates a standardized isolation protocol.

Purpose of the Study:

  • To present a simplified, adaptable protocol for isolating lipid droplets (LDs).
  • To enable broader morphological and biochemical investigations of LDs across various species and tissues.
  • To facilitate advancements in metabolic syndrome research and biofuel development.

Main Methods:

  • A streamlined 7-hour protocol for lipid droplet isolation.
  • Method designed for broad applicability across diverse organisms and tissue types.
  • Minimal optimization required for new sample types.

Main Results:

  • Yields 15-100 μg of protein-equivalent high-quality LDs.
  • Protocol is suitable for a wide range of biological samples.
  • Achieved LDs meet requirements for current research standards.

Conclusions:

  • The developed protocol offers a standardized and accessible approach for LD isolation.
  • This method will accelerate research into the role of LDs in human health and biotechnology.
  • Facilitates the extension of LD studies to underrepresented model organisms and tissues.