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Sequential triplex real-time PCR assay for detecting 21 pneumococcal capsular serotypes that account for a high

Fabiana C Pimenta1, Alexis Roundtree, Ahmet Soysal

  • 1Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Journal of Clinical Microbiology
|December 11, 2012
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...