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Cuixia Dai1, Xiaojing Liu, Shuliang Jiao

  • 1Shanghai Institute of Technology, College of Science, 100 Haiquan Road, Shanghai 201418, China.

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We developed a novel dual-modal imaging system combining spectral domain optical coherence tomography (SD-OCT) and autofluorescence (AF) microscopy. This system enables simultaneous, intrinsically registered imaging of biological samples for enhanced visualization.

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Area of Science:

  • Biomedical Optics
  • Microscopy
  • Optical Imaging

Background:

  • Simultaneous imaging modalities offer complementary information.
  • Spectral domain optical coherence tomography (SD-OCT) provides high-resolution structural information.
  • Autofluorescence (AF) microscopy reveals molecular and metabolic information.

Purpose of the Study:

  • To develop and demonstrate a dual-modal imaging system integrating SD-OCT and AF microscopy.
  • To achieve intrinsically registered images from a single light source.
  • To enable simultaneous structural and molecular contrast imaging of biological tissues.

Main Methods:

  • Utilized a broadband light source centered at 415 nm, generated by frequency-doubling of an ultra-fast Ti:Sapphire laser.
  • Achieved a depth resolution of approximately 12 μm for visible SD-OCT with an 8 nm bandwidth.
  • Integrated SD-OCT and AF microscopy to utilize the same photons for both imaging modalities.

Main Results:

  • Demonstrated simultaneous acquisition of SD-OCT and AF images.
  • Confirmed intrinsic image registration due to the use of identical photons.
  • Successfully imaged biological samples both ex vivo and in vivo.

Conclusions:

  • The developed dual-modal system provides simultaneous OCT imaging and molecular contrasts.
  • Intrinsic image registration simplifies data analysis and interpretation.
  • This technology holds potential for advanced biological and clinical imaging applications.