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Updated: May 16, 2026

Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools
Published on: November 11, 2020
Microscopic structural study of collagen aging in isolated fibrils using polarized second harmonic generation
Dora Aït-Belkacem1, Marie Guilbert, Muriel Roche
1Aix-Marseille Université, Institut Fresnel, CNRS UMR 7249, Ecole Centrale, Marseille, Domaine Universitaire St. Jérôme, 13013 Marseille, France.
Abstract:
Polarization resolved second harmonic generation (PSHG) is developed to study, at the microscopic scale, the impact of aging on the structure of type I collagen fibrils in two-dimensional coatings. A ribose-glycated collagen is also used to mimic tissue glycation usually described as an indicator of aging. PSHG images are analyzed using a generic approach of the molecular disorder information in collagen fibrils, revealing significant changes upon aging, with a direct correlation between molecular disorder and fibril diameters.
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