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Microbial Biosensors01:17

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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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Published on: November 23, 2015

Surface plasmon-based infrared spectroscopy for cell biosensing.

Victor Yashunsky1, Vladislav Lirtsman, Alexander Zilbershtein

  • 1Hebrew University of Jerusalem, The Racah Institute of Physics, 91904 Jerusalem, Israel. victor.yashunsky@mail.huji.ac.il

Journal of Biomedical Optics
|December 11, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a noninvasive technique to monitor cell morphology by measuring infrared reflectivity. The method tracks cell attachment, membrane changes, and junction formation in real-time without labels.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Materials Science

Background:

  • Cell morphology is a key indicator of cell health and function.
  • Existing methods for cell morphology analysis can be invasive or lack real-time capabilities.

Purpose of the Study:

  • To develop a noninvasive, label-free method for real-time monitoring of adherent cell morphology.
  • To utilize surface plasmon and guided modes for sensitive detection of morphological changes.

Main Methods:

  • Measuring infrared reflectivity spectrum at an oblique angle from living cells cultured on a thin gold film.
  • Utilizing confined infrared waves (surface plasmon and guided modes) within the cell layer.
  • Tracking resonant wavelength and attenuation of these modes to infer morphological characteristics.

Main Results:

  • Demonstrated real-time monitoring of submicron variations in cell layer morphology.
  • Successfully measured kinetics of cell attachment, spreading, and membrane modulation.
  • Tracked intercellular junction formation and cell polarization dynamics.

Conclusions:

  • The developed method offers a powerful tool for label-free, real-time assessment of cell morphology and behavior.
  • This technique can provide insights into cellular processes like adhesion, membrane dynamics, and cell-cell interactions.
  • The approach holds potential for various applications in cell biology research and drug discovery.