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Published on: August 6, 2018
Monolithic columns in plant proteomics and metabolomics
Marilda Rigobello-Masini1, José Carlos Pires Penteado, Jorge Cesar Masini
1Instituto de Química, Universidade de São Paulo, C.P. 26077, 05513-970, São Paulo, São Paulo, Brazil. rmmarilda@gmail.com
Analytical and Bioanalytical Chemistry
|December 11, 2012
Summary
Monolithic columns advance plant science by improving proteomics and metabolomics. These advancements enable comprehensive system analysis and prediction of ecosystem changes due to environmental factors.
Area of Science:
- Plant science
- Analytical chemistry
- Biochemistry
- Ecology
Background:
- Omics techniques have revolutionized plant studies, integrating genomics, proteomics, and metabolomics for systems-level understanding.
- Proteomics and metabolomics require advanced separation, identification, and quantification methods for complex biological samples.
- Analytical chemistry plays a crucial role in the evolution of these omics fields.
Purpose of the Study:
- To review recent developments in monolithic column technology for plant proteomics and metabolomics.
- To highlight the applications of monolithic columns in "top-down" and "bottom-up" proteomics.
- To showcase the utility of monolithic columns in analyzing plant metabolites.
Main Methods:
- Utilizing monolithic columns in "top-down" and "bottom-up" proteomic approaches.
- Employing monolithic columns as supports for immobilizing proteolytic enzymes.
- Applying monolithic columns in two-dimensional and multidimensional chromatography.
- Separating proteins, peptides, and small molecules using polymeric and silica-based monolithic columns.
Main Results:
- Polymeric monolithic columns are effective for separating proteins and polypeptides.
- Silica-based monolithic columns show significant utility in separating small molecules and plant metabolites.
- Monolithic columns facilitate efficient separation, identification, and quantification in complex plant samples.
Conclusions:
- Monolithic columns are essential tools for advancing plant proteomics and metabolomics.
- These columns enable comprehensive analysis of plant systems and their responses to environmental perturbations.
- Further development and application of monolithic columns will enhance our understanding of plant biochemistry and ecology.
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