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A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

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An improved dual-tube megaprimer approach for multi-site saturation mutagenesis

Wei Zhang1, Bengt Mannervik

  • 1Department of Chemistry, BMC, Uppsala University, Box 576, 75123 Uppsala, Sweden.

World Journal of Microbiology & Biotechnology
|December 11, 2012
PubMed

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In vitro Mutagenesis01:16

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.