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Updated: May 16, 2026
Modeling Chemotherapy Resistant Leukemia In Vitro
Published on: February 9, 2016
Effects of structural modifications of daunorubicin on in vitro antileukemic activity
Marta Stojak1, Lidia Mazur, Malgorzata Opydo-Chanek
1Department of Experimental Hematology, Jagiellonian University, Gronostajowa 9, 30-387 Cracow, Poland.
Background/Aim:
In the search for new derivatives of anthracycline antibiotics, formamidinodaunorubicins containing in the amidine group either a morpholine moiety (DAUFmor) or a hexamethyleneimine moiety (DAUFhex) were synthesized. The biological effects of daunorubicin (DAU), DAUFmor and DAUFhex were compared.
Materials And Methods:
The experiments were performed on human acute lymphoblastic leukemia MOLT-4 cells and human acute myeloblastic leukemia ML-1 cells. The research was conducted using the spectrophotometric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay and the electronic Beckman-Coulter method.
Results:
Temporary changes in the leukemia cell viability, size and count were found. The antileukemic activities of the new DAU analogs were weaker than that of daunorubicin. MOLT-4 cells were more sensitive than ML-1 cells to the action of all agents. Among the formamidinodaunorubicins, DAUFmor appeared to be more active in ML-1 cells than DAUFhex, but there were not differences between the analyzed values in MOLT-4 cells.
Conclusion:
The structural modifications of daunorubicin were responsible for the different antileukemic potentials of the two formamidinodaunorubicins.
