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10:07
Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
Published on: October 8, 2021
Stabilization of RNA through absorption by functionalized mesoporous silicate nanospheres
Brandy J Johnson1, Brian J Melde, Michael A Dinderman
1Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington DC, USA.
Plos One
|December 11, 2012
Summary
This study shows that porous silica nanoparticles can store and transport RNA, simplifying genetic analysis in field settings. RNA remains stable and usable after elution, reducing the need for cold storage.
Area of Science:
- Biotechnology
- Materials Science
- Molecular Biology
Background:
- Stringent storage requirements for RNA hinder field applications of genetic analysis.
- Developing stable RNA encapsulation methods is crucial for broader accessibility.
Purpose of the Study:
- To evaluate functionalized porous silicate sorbents for maintaining nucleic acid integrity.
- To assess the potential of mesoporous silica nanoparticles (MSNs) for RNA storage and transportation.
Main Methods:
- Encapsulation of Arabidopsis thaliana triosephosphate isomerase mRNA within MSNs.
- Monitoring RNA absorption, elution, and long-term stability using quantitative real-time RT-PCR.
- Utilizing MSNs with and without incorporated stabilizing reagents.
Main Results:
- RNA adsorbed onto MSNs can be eluted using simple buffers for direct use in downstream assays.
- RNA integrity is maintained for extended periods under refrigeration with immobilized stabilizing compounds.
- MSNs demonstrate potential for RNA storage and transportation, even in restrictive environments.
Conclusions:
- Mesoporous silica nanoparticles offer a promising solution for RNA stabilization and transport.
- This method reduces the need for cold chain logistics in genetic analysis.
- MSNs could be valuable for sample collection and processing in challenging field conditions.
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