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Updated: May 16, 2026
06:57
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
Published on: September 18, 2016
Temporal colonic gene expression profiling in the recurrent colitis model identifies early and chronic inflammatory
Bas Kremer1, Rob Mariman, Marjan van Erk
1Department of Metabolic Health Research, TNO, Leiden, The Netherlands. bas.kremer@tno.nl
Plos One
|December 11, 2012
Summary
This study shows the recurrent TNBS-colitis mouse model mimics inflammatory bowel disease (IBD) pathology, with immune responses changing over time. Early stages are sensitive to budesonide treatment, indicating potential therapeutic avenues.
Area of Science:
- Gastroenterology
- Immunology
- Pathology
Background:
- The TNBS-colitis model in BALB/c mice is used to study Inflammatory Bowel Disease (IBD).
- IBD models exhibit shifting cytokine patterns (Th1, Th17, Th2) during disease progression.
Purpose of the Study:
- To evaluate the temporal development of pathology and gene expression in the TNBS-colitis model.
- To assess the impact of budesonide treatment on colitis development.
Main Methods:
- Induction of recurrent colitis via intrarectal TNBS instillations in mice.
- Histopathological examination of colon tissue.
- Genome-wide gene expression profiling of colon tissue.
Main Results:
- TNBS-induced colitis showed cellular infiltration and crypt architecture damage.
- Gene expression confirmed acute phase response and a chronic inflammatory process with increased mast cell-related genes.
- Pathological changes correlated with temporal mRNA expression of chemokines.
- Budesonide treatment demonstrated sensitivity in managing pathological processes.
Conclusions:
- The early stages of the recurrent TNBS-colitis model share key features with human IBD.
- These early inflammatory aspects are responsive to immunomodulatory treatment.
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