Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complement System01:27

Complement System

The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a membrane...
Complementation Tests00:49

Complementation Tests

A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
Pneumonia III: Complications and Assessment01:30

Pneumonia III: Complications and Assessment

Pneumonia poses the potential for numerous complications that warrant consideration. These complications include the following:
Sensitivity, Specificity, and Predicted Value01:13

Sensitivity, Specificity, and Predicted Value

In healthcare diagnostics, laboratory tests play a crucial role in identifying and diagnosing a wide range of medical conditions. However, interpreting test results is not always straightforward. An abnormal test result does not always confirm the presence of a disease, just as a normal result does not guarantee its absence. To assess the reliability of these diagnostic tools, healthcare practitioners rely on two key statistical indicators: sensitivity and specificity.
Sensitivity is the...
Receiver Operating Characteristic Plot01:15

Receiver Operating Characteristic Plot

A ROC (Receiver Operating Characteristic) plot is a graphical tool used to assess the performance of a binary classification model by illustrating the trade-off between sensitivity (true positive rate) and specificity (false positive rate). By plotting sensitivity against 1 - specificity across various threshold settings, the ROC curve shows how well the model distinguishes between classes, with a curve closer to the top-left corner indicating a more accurate model. The area under the ROC curve...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vivo evidence of effective complement modulation by TriFu in comparison to C5 inhibition and complement depletion.

Blood advances·2026
Same author

The Cellular Response Capacity as Diagnostic Head Start in Neutrophil Endotoxemia Sensing.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Reply to Comment on "A new principle to attenuate ischemia-reperfusion injury in kidney transplantation".

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Vulnerabilities, extreme weather and temporal tensions as experienced by construction workers in the Swedish construction sector.

PloS one·2026
Same author

Nuclear cell-free DNA on the loose: an early warning signal of ischemia-reperfusion injury in kidney transplantation.

Frontiers in immunology·2026
Same author

Native C3 is activated without proteolytic cleavage by transformation to C3(H<sub>2</sub>O) on phospholipid-scrambled cell membranes.

Frontiers in immunology·2026
Same journal

Cell-mediated immune responses in paraneoplastic neurological syndromes.

Clinical & developmental immunology·2014
Same journal

Contrasting effects of systemic monocyte/macrophage and CD4+ T cell depletion in a reversible ureteral obstruction mouse model of chronic kidney disease.

Clinical & developmental immunology·2014
Same journal

Hepatic expression of metallothionein I/II, glycoprotein 96, IL-6, and TGF- β in rat strains with different susceptibilities to experimental autoimmune encephalomyelitis.

Clinical & developmental immunology·2014
Same journal

The role of cytokines in the functional activity of phagocytes in blood and colostrum of diabetic mothers.

Clinical & developmental immunology·2014
Same journal

Immunology and the central nervous system.

Clinical & developmental immunology·2014
Same journal

Persistence of Th17/Tc17 cell expression upon smoking cessation in mice with cigarette smoke-induced emphysema.

Clinical & developmental immunology·2014
See all related articles

Related Experiment Video

Updated: May 16, 2026

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
06:29

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

Complement diagnostics: concepts, indications, and practical guidelines.

Bo Nilsson1, Kristina Nilsson Ekdahl

  • 1Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Sweden. bo.nilsson@igp.uu.se

Clinical & Developmental Immunology
|December 11, 2012
PubMed
Summary
This summary is machine-generated.

Complement system analysis aids in diagnosing diseases like autoimmune disorders and infections. Current assays lack sensitivity for individual patient predictions, necessitating improved diagnostic strategies.

More Related Videos

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
08:26

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum

Published on: March 29, 2010

Depletion of Specific Cell Populations by Complement Depletion
06:17

Depletion of Specific Cell Populations by Complement Depletion

Published on: February 5, 2010

Related Experiment Videos

Last Updated: May 16, 2026

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
06:29

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
08:26

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum

Published on: March 29, 2010

Depletion of Specific Cell Populations by Complement Depletion
06:17

Depletion of Specific Cell Populations by Complement Depletion

Published on: February 5, 2010

Area of Science:

  • Immunology
  • Clinical Diagnostics

Background:

  • Complement system aberrations are implicated in various diseases, including autoimmune disorders, infections, cancer, transplantation, and inflammation.
  • While complement analyses reveal group differences, current assays often lack the sensitivity and specificity for individual patient prognostication.

Purpose of the Study:

  • To summarize the indications, techniques, and interpretations for basic complement analyses.
  • To present a routine laboratory algorithm for complement diagnostics.

Main Methods:

  • Review of indications for serological diagnostic complement analysis.
  • Description of techniques and interpretation guidelines for basic complement assays.
  • Presentation of a laboratory diagnostic algorithm.

Main Results:

  • Basic indications for complement analysis fall into three categories: deficiencies, activation disorders, and C1 inhibitor deficiencies.
  • Current assays show limitations in sensitivity and specificity for individual patient predictions.
  • A structured algorithm aids in the routine interpretation of complement analyses.

Conclusions:

  • Standardized complement analysis indications and interpretation are crucial for diagnosing complement-related disorders.
  • Improved assay sensitivity and specificity are needed for precise individual patient management.
  • The presented algorithm provides a framework for consistent and effective complement diagnostics.