Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

2° Amines to N-Nitrosamines: Reaction with NaNO2

2° Amines to N-Nitrosamines: Reaction with NaNO₂

Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The volatile organic compounds generated from the Maillard reaction between l-ascorbic acid and l-cysteine in hot compressed water.

Journal of the science of food and agriculture·2024

Same author

Long-term outcomes and independent predictors of mortality in patients presenting to emergency departments with acute heart failure in Beijing: a multicenter cohort study with a 5-year follow-up.

Chinese medical journal·2021

Same author

[Progress in the effects of injury and regeneration of gustatory nerves on the taste functions in animals].

Sheng li xue bao : [Acta physiologica Sinica]·2014

Same author

[Advances of chemical constituents and pharmacological activities of Myristica genus].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·2014

Same author

Effect of siRNA‑induced inhibition of IL‑6 expression in rat cerebral gliocytes on cerebral edema following traumatic brain injury.

Molecular medicine reports·2014

Same author

Effects of chronic cold exposure on murine central nervous system.

Journal of neuroscience research·2014

Same journal

[The protective effects of curcumin loaded mesoporous silica nanoparticles on rat cardiomyocytes].

Yao xue xue bao = Acta pharmaceutica Sinica·2018

Same journal

[Identification of water buffalo horn and its adulterants using COI barcode].

Yao xue xue bao = Acta pharmaceutica Sinica·2018

Same journal

[Construction of the brain-targeting drug carrier through imprinting of nicotinic acetylcholine receptor α7].

Yao xue xue bao = Acta pharmaceutica Sinica·2018

Same journal

[Application of magnetic iron oxide nanoparticles in magnetic resonance / photothermal dual-modal imaging].

Yao xue xue bao = Acta pharmaceutica Sinica·2018

Same journal

[Endocytosis pathway and intracellular distribution of heparosan].

Yao xue xue bao = Acta pharmaceutica Sinica·2018

Same journal

[On-line detection of concentration process of Ganmaoling granules by near infrared spectroscopy combined with automatic control system].

Yao xue xue bao = Acta pharmaceutica Sinica·2018

See all related articles

Search research articles

Related Experiment Video

Updated: May 16, 2026

Designing Porous Silicon Films as Carriers of Nerve Growth Factor

Designing Porous Silicon Films as Carriers of Nerve Growth Factor

Published on: January 25, 2019

[Sodium nitrite induces PC12 cell differentiation].

Wen-Yi Yan¹, Dong-Ming Yu, Chao-Shen Huang-Fu

¹Institute of Environmental Medicine, Medical College of Henan University, Kaifeng 475004, China.

Yao Xue Xue Bao = Acta Pharmaceutica Sinica

|December 11, 2012

Summary

This summary is machine-generated.

Low doses of sodium nitrite promote neuronal differentiation in PC12 cells by stimulating neurite outgrowth. This effect is mediated through the hypoxia-inducible factor 1-alpha (HIF-1α) and vascular endothelial growth factor (VEGF) pathway.

More Related Videos

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact

Published on: July 17, 2016

Related Experiment Videos

Last Updated: May 16, 2026

Designing Porous Silicon Films as Carriers of Nerve Growth Factor

Designing Porous Silicon Films as Carriers of Nerve Growth Factor

Published on: January 25, 2019

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact

Published on: July 17, 2016

Area of Science:

Neuroscience
Cell Biology
Biochemistry

Background:

Neuronal differentiation is crucial for nervous system development and repair.
PC12 cells are a widely used model for studying neuronal differentiation.
Identifying novel compounds that can induce neuronal differentiation is of significant therapeutic interest.

Purpose of the Study:

To investigate the potential of sodium nitrite to induce neuronal differentiation in PC12 cells.
To elucidate the underlying molecular mechanisms, including the role of hypoxia-inducible factor 1-alpha (HIF-1α) and vascular endothelial growth factor (VEGF).

Main Methods:

PC12 cells were cultured on matrigel with or without sodium nitrite.
Nerve growth factor (NGF) was used as a positive control.
Cell viability, neurite outgrowth, and the expression of VEGF mRNA and protein were assessed.
The involvement of HIF-1α was investigated using an inhibitor (YC-1).

Main Results:

Sodium nitrite significantly enhanced PC12 cell viability and vascular endothelial growth factor (VEGF) secretion.
Sodium nitrite treatment led to a greater proportion of cells with longer neurites, similar to NGF.
The expression of VEGF mRNA and HIF-1α/VEGF protein was upregulated by sodium nitrite.
The effects of sodium nitrite were antagonized by the HIF-1α inhibitor YC-1, suggesting mediation by HIF-1α.

Conclusions:

Low doses of sodium nitrite can induce neurite outgrowth in PC12 cells.
This induction appears to be mediated by the activation of the HIF-1α-VEGF pathway.
Sodium nitrite shows potential as an agent for promoting neuronal differentiation.