Related Experiment Video

Updated: May 16, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Effects of siRNA‑mediated Cdc2 silencing on MG63 cell proliferation and apoptosis

Xiang-Yong Que1, Yi Li, Yu Han

  • 1Department of Orthopedics, Renhe Hospital of Three Gorges University, Yichang 443001, P.R. China.

Molecular Medicine Reports
|December 12, 2012
PubMed

Insights

Silencing cyclin-dependent kinase 2 (Cdc2) with siRNA inhibits osteosarcoma cell proliferation by altering the cell cycle. This study found Cdc2 inhibition impacts cell cycle phases but not apoptosis-related molecules.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Biology

Background:

  • Osteosarcoma is a prevalent bone cancer.
  • Cyclin-dependent kinase 2 (Cdc2) plays a role in cell cycle regulation.
  • Targeting Cdc2 may offer a therapeutic strategy for osteosarcoma.

Purpose of the Study:

  • To investigate the effect of inhibiting cyclin-dependent kinase 2 (Cdc2) expression on osteosarcoma MG63 cell proliferation and apoptosis.
  • To evaluate the efficacy of small interfering RNA (siRNA) in silencing Cdc2 expression in MG63 cells.

Main Methods:

  • Osteosarcoma MG63 cells were transfected with siRNA targeting Cdc2 (psilencer 2.1‑U6/Cdc2) or a scrambled control.
  • Cdc2 expression was measured using western blot and real-time PCR.
  • Cell proliferation, cell cycle, and apoptosis-related molecules (Bcl-2, Bax) were assessed via MTT assay, flow cytometry, and western blot.

Main Results:

  • Successful establishment of MG63 cells with silenced Cdc2 expression (MG63‑siRNA/Cdc2).
  • Silencing efficiency for Cdc2 mRNA and protein reached 86% and 89%, respectively.
  • Inhibition of Cdc2 suppressed MG63 cell proliferation, increased G2/M phase cells, and decreased S phase cells.
  • Cdc2 silencing showed negligible impact on Bcl-2 and Bax expression.

Conclusions:

  • Silencing Cdc2 expression effectively suppresses osteosarcoma MG63 cell proliferation.
  • Cdc2 inhibition influences the cell cycle distribution in osteosarcoma cells.
  • Cdc2 silencing does not significantly affect apoptosis-related gene expression in this model.

More Related Videos

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures07:48

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures

Published on: December 26, 2016

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity04:20

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity

Published on: February 9, 2024

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...