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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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Related Experiment Video

Updated: May 16, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

RNA helicases in splicing.

Olivier Cordin¹, Jean D Beggs

¹Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK.

|December 12, 2012

Summary

This summary is machine-generated.

This review details the dynamic roles of eight DExD/H RNA helicases in spliceosome function. Understanding these splicing helicase interactions is key to comprehending normal and pathological RNA splicing.

Keywords:

ATPase DEAD-box DEAH-box DExD/H helicase protein partners

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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Published on: April 22, 2021

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Last Updated: May 16, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

Molecular Biology
RNA Biology
Cellular Mechanisms

Background:

The spliceosome, a dynamic molecular machine, orchestrates pre-mRNA splicing in eukaryotic cells.
Eight DExD/H RNA helicases are crucial for regulating spliceosome structural dynamics and function.
Interactions among helicases and other spliceosomal factors are vital for splicing regulation.

Purpose of the Study:

To review recent advancements in characterizing splicing helicases and their interactions.
To elucidate the dynamic nature of spliceosome composition and structure.
To highlight the integration of splicing helicases within messenger ribonucleoprotein (mRNP) biogenesis.

Main Methods:

Literature review of recent studies on splicing helicases.
Analysis of structural and functional data regarding spliceosome dynamics.
Integration of findings on helicase-factor interactions and mRNP pathways.

Main Results:

Splicing helicases exhibit complex functional and physical interconnections within the spliceosome.
These helicases are deeply integrated into global messenger ribonucleoprotein (mRNP) biogenesis.
Understanding helicase dynamics is essential for elucidating splicing mechanisms and regulation.

Conclusions:

Recent research has significantly advanced our understanding of splicing helicase roles.
The dynamic interactions of splicing helicases are central to both normal and aberrant splicing processes.
Splicing helicases are integral components of broader mRNA processing and biogenesis pathways.