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Related Concept Videos

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...

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Related Experiment Video

Updated: May 16, 2026

Yeast Colony Embedding Method
09:04

Yeast Colony Embedding Method

Published on: March 22, 2011

Visualization of yeast cells by electron microscopy.

Masako Osumi1

  • 1Laboratory of Electron Microscopy/Bio-imaging Center, Japan Women's University, 2-8-1 Mejirodai, Bunkyo-ku, Tokyo, Japan. osumi@fc.jwu.ac.jp

Journal of Electron Microscopy
|December 13, 2012
PubMed
Summary
This summary is machine-generated.

Yeast physiology studies reveal microbody biogenesis and cell wall formation. Researchers identified key enzymes and glucans in yeast cell walls using advanced microscopy techniques for ultrastructure research.

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A Rapid Technique for the Visualization of Live Immobilized Yeast Cells
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Published on: November 9, 2006

Area of Science:

  • Microbiology
  • Cell Biology
  • Biochemistry

Background:

  • Yeast, particularly hydrocarbon or methanol-utilizable strains, were historically explored for food and ethanol production.
  • Physiological studies of yeasts revealed their utility as model systems for investigating microbody (peroxisome) biogenesis and ultrastructure.
  • Previous research indicated that microbodies in hydrocarbon-utilizing yeasts like Candida tropicalis proliferate from existing structures.

Purpose of the Study:

  • To investigate the biogenesis and ultrastructure of yeast microbodies.
  • To identify and characterize enzymes within microbodies using immunoelectron microscopy.
  • To elucidate the composition and formation process of the yeast cell wall and septum during cell division.

Main Methods:

  • Immunoelectron microscopy to determine β-oxidation enzymes in Candida tropicalis microbodies.
  • Optical diffraction, filtering, and computer simulation to analyze the ultrastructure of Candida boidinii microbodies.
  • Protoplast preparation and regeneration in Schizosaccharomyces pombe, coupled with ultra-high resolution field emission scanning electron microscopy (UHR-FESEM).
  • Cryo-fixation by high-pressure freezing (HPF) for examining septum formation.
  • Focused ion beam (FIB) microscopy and scanning transmission electron microscopy (STEM) for 3D imaging and molecular architecture analysis.

Main Results:

  • Microbodies in Candida tropicalis multiply from preexisting ones, with β-oxidation enzymes localized within.
  • Candida boidinii microbodies contain a composite crystalloid of alcohol oxidase and catalase.
  • The yeast cell wall is composed of β-1,3-glucan, β-1,6-glucan, α-1,3-glucan, and α-galactomannan.
  • During septum formation, α-1,3- and β-1,3-glucans are present in the nascent septum, followed by β-1,6-glucan in the second septum.

Conclusions:

  • Yeast systems offer valuable models for microbody biogenesis and ultrastructure research.
  • Advanced electron microscopy techniques, including FIB and STEM, are powerful tools for analyzing cellular molecular architecture and enabling electron tomography.
  • The study successfully detailed the composition of yeast cell walls and the dynamic process of septum formation during cell division.