Related Experiment Video
Updated: Jul 21, 2026
06:30
Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM
Published on: March 2, 2017
Localization of the human A1S9 gene complementing the ts A1S9 mouse L-cell defect in DNA replication and cell cycle
E Zacksenhaus1, R Sheinin, H S Wang
1Department of Microbiology, Faculty of Medicine, University of Toronto, Ontario, Canada.
Cytogenetics and Cell Genetics
|January 1, 1990
Summary
Researchers identified a gene crucial for cell cycle progression in a mouse mutant. This gene was localized to a specific region on the human X chromosome, aiding in understanding cell division defects.
Area of Science:
- Genetics
- Cell Biology
- Molecular Biology
Background:
- A temperature-sensitive mouse L-cell mutant (ts A1S9) exhibits a defect in an X-linked gene essential for S phase progression during the cell division cycle.
- Understanding the genetic basis of cell cycle regulation is critical for comprehending normal development and diseases like cancer.
Purpose of the Study:
- To identify and localize the human gene responsible for complementing the ts A1S9 mouse mutation.
- To determine the precise chromosomal location of the human A1S9 gene.
Main Methods:
- Utilized a DNA fragment from the human A1S9 gene as a probe for gene localization.
- Employed Southern blot analysis with human x rodent somatic cell hybrids.
- Performed in situ hybridization to human metaphase chromosomes.
Main Results:
- The human A1S9 gene was successfully mapped to the X chromosome.
- Regional assignment of the human A1S9 gene was determined to be Xp11.2-p11.4 using molecular and cytogenetic techniques.
Conclusions:
- The human A1S9 gene, essential for S phase progression, is located on the X chromosome at region Xp11.2-p11.4.
- This localization provides a crucial step towards the molecular characterization of this cell cycle regulatory gene and its potential role in human genetic disorders.
