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New amide alkaloids from Piper longum.

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Two new compounds were isolated from Piper longum. Compound 1 showed significant anti-HBV activity by suppressing HBeAg secretion in liver cells.

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Area of Science:

  • Natural Product Chemistry
  • Medicinal Chemistry
  • Hepatology

Background:

  • Hepatitis B virus (HBV) infection is a global health concern.
  • Developing novel antiviral agents is crucial for HBV treatment.
  • Piper longum is a medicinal plant with a history of traditional use.

Purpose of the Study:

  • To isolate and characterize new compounds from Piper longum.
  • To evaluate the anti-HBV activity of the isolated compounds.

Main Methods:

  • Bioassay-guided fractionation of Piper longum extract.
  • Structure elucidation using spectroscopic techniques (MS, IR, UV, 1D and 2D NMR).
  • In vitro anti-HBV assay using Hep G2.2.15 cell line to measure HBeAg secretion.

Main Results:

  • Two new compounds were identified: 3β, 4α-dihydroxy-1-(3-phenylpropanoyl)-piperidine-2-one (1) and (2E, 4E, 14Z)-6-hydroxyl-N-isobutyleicosa-2,4,14-trienamide (2).
  • Compound 1 demonstrated significant activity in suppressing HBeAg secretion.
  • Compound 1 exhibited an IC(50) value of 0.21mM and a Selectivity Index (SI) of 16.4.

Conclusions:

  • Compound 1, a novel piperidine derivative from Piper longum, possesses potent anti-HBV activity.
  • This compound warrants further investigation as a potential therapeutic agent for Hepatitis B.
  • Piper longum is a valuable source of bioactive compounds with antiviral properties.