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Updated: May 16, 2026

Bone Conditioned Medium: Preparation and Bioassay
Published on: July 8, 2015
An overview on bone protein extract as the new generation of demineralized bone matrix
Zhiyu Zhou1, Lijin Zou, Haisheng Li
1Orthopaedic Research Institute/Department of Orthpaedic Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
Abstract:
Bone protein extract is regarded as the new generation of demineralized bone matrix. The aim of this paper is to describe and characterize the properties of demineralized bone matrix and its new generation product in addition to its application in animal and human studies. Bone protein extract has features of osteoconductivity, osteoinductivity and osteogenicity, which originate from its unique and precise processing. It has exhibited powerful bone formation capacity both in animal experiments and in clinical trials by providing an optimal microenvironment for osteogenesis. Furthermore, not only does it have excellent biocompatibility, it also has good compatibility with other implant materials, helping it bridge the host and implanted materials. Bone protein extract could be a promising alternative for demineralized bone matrix as a bone graft substitute.
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