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Characterizing inter-compartmental water exchange in myelinated tissue using relaxation exchange spectroscopy
Richard D Dortch1, Kevin D Harkins, Meher R Juttukonda
1Department of Radiology and Radiological Sciences, Vanderbilt University, Nashville, Tennessee, USA; Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, Tennessee, USA.
Purpose:
To investigate inter-compartmental water exchange in two model myelinated tissues ex vivo using relaxation exchange spectroscopy.
Methods:
Building upon a previously developed theoretical framework, a three-compartment (myelin, intra-axonal, and extra-axonal water) model of the inversion-recovery prepared relaxation exchange spectroscopy signal was applied in excised rat optic nerve and frog sciatic nerve samples to estimate the water residence time constants in myelin (τmyelin ).
Results:
In the rat optic nerve samples, τmyelin = 138 ± 15 ms (mean ± standard deviation) was estimated. In sciatic nerve, which possesses thicker myelin sheaths than optic nerve, a much longer τmyelin = 2046 ± 140 ms was observed.
Conclusion:
Consistent with previous studies in rat spinal cord, the extrapolation of exchange rates in optic nerve to in vivo conditions indicates that τmyelin < 100 ms. This suggests that there is a significant effect of inter-compartmental water exchange on the transverse relaxation of water protons in white matter. The much longer τmyelin values in sciatic nerve supports the postulate that the inter-compartmental water exchange rate is mediated by myelin thickness. Together, these findings point to the potential for MRI methods to probe variations in myelin thickness in white matter.
