Related Experiment Video
Updated: May 16, 2026
07:16
High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC
Published on: February 2, 2024
[Chemical constituents from Hypericum perforatum].
Jie Ma1, Jianbo Yang, Tengfei Ji
1Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Summary
Researchers identified new chemical compounds from Hypericum perforatum. One compound, hypericin (8), demonstrated significant protein tyrosine phosphatase 1B (PTP1B) inhibitory activity, suggesting therapeutic potential.
Area of Science:
- Phytochemistry
- Natural Product Chemistry
- Pharmacology
Background:
- Hypericum perforatum, commonly known as St. John's Wort, is a medicinal plant with a long history of use.
- Understanding its chemical composition is crucial for elucidating its pharmacological activities and potential therapeutic applications.
- Protein tyrosine phosphatase 1B (PTP1B) is a key target for metabolic diseases like diabetes and obesity.
Purpose of the Study:
- To isolate and identify chemical constituents from Hypericum perforatum.
- To evaluate the inhibitory activity of isolated compounds against recombinant human PTP1B.
Main Methods:
- Compounds were isolated using various chromatographic techniques.
- Structural elucidation was performed using advanced spectral methods (e.g., NMR, Mass Spectrometry).
- In vitro enzyme inhibition assays were conducted to assess PTP1B inhibitory activity.
Main Results:
- Ten compounds were identified, including D-Mannitol, esters, glycosides, and known compounds like hypericin.
- Compounds 2-7 were reported as new for Hypericum perforatum, with compound 10 being a novel chemical entity.
- Hypericin (compound 8) exhibited potent inhibition of recombinant human PTP1B, with an inhibitory rate of 96.4% and an IC50 of 2.5 µmol/L at 2 µmol/L concentration.
Conclusions:
- The study successfully isolated and characterized several chemical constituents from Hypericum perforatum.
- Compound 10 represents a newly discovered chemical structure.
- Hypericin (compound 8) demonstrated significant PTP1B inhibitory activity, highlighting its potential as a therapeutic agent for PTP1B-related disorders.
Related Concept Videos
01:29Spasmolytic Agents: Chemical Classification
Spasmolytic agents are drugs used to alleviate muscle spasms and spasticity. They can be categorized into different chemical groups based on their mechanisms of action. Centrally acting spasmolytics primarily affect the spinal cord, while others directly target skeletal muscle cells.
A major class of centrally acting spasmolytics is the α2-agonist, such as tizanidine. These drugs bind to α2-adrenoceptors, inhibiting the release of the excitatory neurotransmitter glutamate. They also promote...
A major class of centrally acting spasmolytics is the α2-agonist, such as tizanidine. These drugs bind to α2-adrenoceptors, inhibiting the release of the excitatory neurotransmitter glutamate. They also promote...
01:27Effects of Chemicals: Overview
Drugs, encompassing various chemical compounds from natural sources, lab synthesis, or genetic engineering, elicit different biological responses in living organisms. Some of these responses are desirable or therapeutic, while others are undesirable. The primary goal of administering a drug is to achieve a therapeutic effect, that is, to address a specific disease or health condition. Any concurrent effects outside of this therapeutic outcome are considered undesirable. These undesirable...
01:30Methods of Sterilization II: Chemical Methods
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
01:20Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry
Orally administered drugs primarily enter the systemic circulation via passive diffusion through the intestinal membranes. The drug's absorption is influenced by drug stability in the gastrointestinal GI tract, membrane permeability, the surface area available for absorption, luminal drug concentration, and residence time in the lumen. Drug permeability can be enhanced by adjusting the lipophilicity, polarity, or molecular size of the drug, promoting its passive transport across intestinal...
01:31Hormones of the Adrenal Glands
Adrenal hormones play a pivotal role in maintaining the body's electrolyte balance and orchestrating responses to stress, showcasing the intricate functions of the adrenal cortex and medulla.
The adrenal cortex, a powerhouse of hormone synthesis, generates over two dozen corticosteroid hormones. The zona glomerulosa produces mineralocorticoids, exemplified by aldosterone, influencing the electrolyte composition of body fluids. The synthesis of glucocorticoids such as cortisol and corticosterone...
The adrenal cortex, a powerhouse of hormone synthesis, generates over two dozen corticosteroid hormones. The zona glomerulosa produces mineralocorticoids, exemplified by aldosterone, influencing the electrolyte composition of body fluids. The synthesis of glucocorticoids such as cortisol and corticosterone...
01:25Adrenergic Antagonists: Chemistry and Classification of β-Receptor Blockers
β-adrenergic antagonists, or β-blockers, modulate the sympathetic nervous system by targeting β-adrenoceptors and inhibiting catecholamine-mediated sympathetic responses. β-blockers differ in their adrenoceptor subtype affinity, lipophilicity, and α-blocking capabilities. The history of β-blocker development began with the prototype, dichloroisoprenaline, which exhibited partial agonist activity. As a result, propranolol was developed as a pure antagonist but nonselective agent, paving the way...