Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cadherins in Tissue Organization

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...

Tissue-Drug Binding: Localization of Drugs and its Significance

Tissue-Drug Binding: Localization of Drugs and its Significance

Body tissues, comprising approximately 40% of the body weight, are crucial in drug distribution and localization. These tissues can serve as drug storage sites, competing with plasma binding sites for drug molecules.
Drugs can bind to different tissue components, enhancing their distribution and localization. The factors influencing drug localization in tissues include the drug's lipophilicity, structural characteristics, tissue perfusion rate, and pH differences. These factors determine the...

Transducer Mechanism: Nuclear Receptors

Transducer Mechanism: Nuclear Receptors

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Role and relevance of genetic testing in patients with kidney stones: a review from EAU Section of Endourology.

Current opinion in urology·2026

Same author

Long-term citrate treatment in high-risk kidney stone formers is not associated with metabolic adverse effects.

Clinical kidney journal·2026

Same author

Effect of Sodium Bicarbonate on Indicators of Volume Retention in Metabolic Acidosis After Kidney Transplantation: A Post-Hoc Analysis.

Kidney medicine·2026

Same author

CO and N<sub>2</sub> are not humane alternatives to CO<sub>2</sub> euthanasia in rats.

Pflugers Archiv : European journal of physiology·2026

Same author

Deep biochemical phenotyping reveals prognostic value of rare genetic variants in adult kidney stone disease.

The Journal of clinical investigation·2026

Same author

A Large Deletion With a Large Impact: Homozygous 5,600 bp Deletion of the <i>GALNT3</i> Gene Causing Hyperphosphatemic Tumoral Calcinosis.

Kidney medicine·2026

Search research articles

Related Experiment Video

Updated: May 16, 2026

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

DOCA sensitive pendrin expression in kidney, heart, lung and thyroid tissues.

Lisann Pelzl¹, Tatsiana Pakladok, Ganesh Pathare

¹Department of Physiology, University of Tuebingen, Tuebingen, Germany.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology

|December 14, 2012

Summary

Mineralocorticoids like DOCA increase pendrin (SLC26A4) expression in the kidneys, heart, lungs, and thyroid. This finding reveals the sensitivity of pendrin to mineralocorticoids in these vital organs.

More Related Videos

Accessing the Cytotoxicity and Cell Response to Biomaterials

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

Related Experiment Videos

Last Updated: May 16, 2026

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

Accessing the Cytotoxicity and Cell Response to Biomaterials

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

Area of Science:

Physiology
Molecular Biology
Endocrinology

Background:

Pendrin (SLC26A4) is an anion transporter found in various organs, including the inner ear, thyroid, kidneys, lung, liver, and heart.
Mutations in SLC26A4 cause Pendred syndrome, leading to hearing loss and sometimes hypothyroidism.
While renal pendrin expression is known to be upregulated by mineralocorticoids, its regulation in other tissues remains unclear.

Purpose of the Study:

To investigate the impact of mineralocorticoids on pendrin expression in extrarenal tissues.
To determine if mineralocorticoids influence pendrin levels in the kidney, thyroid, heart, and lung.

Main Methods:

Quantitative real-time PCR (RT-qPCR) was used to measure Slc26a4 transcript levels.
Western blotting was employed to assess pendrin protein abundance.
These analyses were performed on murine kidney, thyroid, heart, and lung tissues before and after administration of deoxycorticosterone (DOCA).

Main Results:

DOCA treatment significantly increased Slc26a4 transcript levels in the kidney, heart, lung, and thyroid compared to Gapdh.
Pendrin protein expression was also significantly elevated by DOCA treatment across all investigated tissues (kidney, heart, lung, and thyroid).

Conclusions:

Pendrin expression in the kidney, heart, thyroid, and lung is sensitive to mineralocorticoids.
These findings highlight a broader role for mineralocorticoids in regulating pendrin expression beyond the kidney.