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Diisopropylfluorophosphate-binding proteins in the outer membrane of Fusobacterium nucleatum: strain variations
K A Brokstad1, V Bakken, E N Vasstrand
1Department of Biochemistry, University of Bergen, Norway.
FEMS Microbiology Letters
|January 1, 1990
Abstract:
Six strains of Fusobacterium nucleatum were tested for their ability to react with [3H]diisopropylfluorophosphate (DFP), a serine protease inhibitor. Several cytoplasmic proteins were labelled but the strongest labelling was regularly observed in a few outer membrane proteins. The number and the molecular mass of the proteins detected varied according to the strain tested. A 61 kDa protein was labelled in all strains tested, including the two type strains ATCC 10953 and ATCC 25586. A 65 kDa protein was particularly strongly labelled in strains Fev1 and F6.