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Related Experiment Videos

Cloning and sequence analysis of the human gene encoding eosinophil major basic protein.

R L Barker1, D A Loegering, K C Arakawa

  • 1Department of Immunology, Mayo Medical School, Rochester, MN.

Gene
|February 14, 1990
PubMed
Summary

The eosinophil major basic protein (MBP) gene was cloned and sequenced. This study details the gene

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Area of Science:

  • Molecular Biology
  • Immunology
  • Genetics

Background:

  • Eosinophils are immune cells that release major basic protein (MBP).
  • MBP is a potent toxin effective against helminths and mammalian cells.
  • MBP is a single polypeptide characterized by its high arginine content.

Purpose of the Study:

  • To clone and determine the nucleotide sequence of the eosinophil major basic protein (MBP) gene.
  • To analyze the structural features of the MBP gene, including exons, introns, and regulatory elements.
  • To investigate the polymorphism of the MBP gene.

Main Methods:

  • Gene cloning techniques were employed to isolate the MBP gene.
  • Nucleotide sequencing was performed to determine the genetic sequence.

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  • Southern blotting was used to assess gene polymorphism.

    • Main Results:

    • The 3.3-kb MBP gene was successfully cloned and its nucleotide sequence determined.
    • The gene comprises six exons and five introns, with one intron containing an Alu family repeat.
    • The deduced exon sequence aligns with previously reported MBP cDNA sequences.
    • Analysis revealed a putative promoter region with TATA and CCAAT boxes preceding the gene.
    • Southern blot analysis indicated limited polymorphism in the MBP gene.

    Conclusions:

    • The complete nucleotide sequence and structural organization of the eosinophil MBP gene have been elucidated.
    • The identified promoter elements suggest transcriptional regulation mechanisms.
    • The findings provide a foundation for further studies on MBP gene expression and function.